|Product Overview:||Recombinant Human SIGLEC15(Phe20-Thr263) fused Fc region of Human IgG1 at C-terminal was expressed in CHO.|
|Description:||Siglec-15 is a transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules. Mature human Siglec-15 consists of a 244 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain. Within the ECD, human Siglec-15 shares 85% aa sequence identity with mouse and rat Siglec-15. Alternative splicing generates an additional isoform that lacks the signal peptide and first Ig-like domain. Siglec-15 associates with the signaling molecules DAP12 and DAP10. It is expressed on osteoclasts, macrophages, and dendritic cells and binds to the sialyl-Tn antigen. This interaction induces the production of TGF-beta by tumor-associated macrophages. Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts.|
|Predicted N Terminal:||Phe20|
|Form:||Lyophilized from a 0.2 μm filtered solution in PBS, NaCl, CHAPS and Trehalose.|
|Bio-activity:||Measured by its binding ability in a functional ELISA. When Neu5Ac(a2-6)GalNAc-PAA-biotin is immobilized onto a Streptavidin-coated plate, Recombinant Human Siglec-15 Fc Chimera binds with an ED50 of 150-900 ng/mL.|
|Molecular Mass:||Predicted Molecular Mass: 53 kDa
SDS-PAGE: 53-64 kDa, reducing conditions
|Endotoxin:||<0.1 EU per 1 μg of the protein by the LAL method.|
|Purity:||>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.|
|Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
|Reconstitution:||Reconstitute at 200 μg/mL in water.|
|Gene Name:||SIGLEC15 sialic acid binding Ig like lectin 15 [ Homo sapiens ]|
|Synonyms:||CD33L3; HsT1361; SIGLEC-15; CD33 antigen-like 3; CD33 molecule-like 3|
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