Recombinant Human SMAD3 protein, GST-tagged
Cat.No. : | SMAD3-295H |
Product Overview : | Recombinant Human SMAD3(C-terminal SXS deletion) fused with GST tag at N-terminal was expressed in E. coli. |
- Specification
- Gene Information
- Related Products
Description : | SMAD3 is a direct mediator of transcriptional activation by the TGF receptor. The activity of SMAD3 is regulated by the TGF receptors, and SMAD3 is phosphorylated and associated with the ligand-bound receptor complex. TGF stimulation leads to phosphorylation and activation of SMAD3, which form a complex with SMAD4 that accumulate in the nucleus and regulate transcription of target genes such as CDK inhibitor. SMAD3 containing a C-terminal truncation acts as a dominant-negative inhibitor of the normal TGF response. SMAD3 is a major physiologic substrate of the G1 cyclin-dependent kinases CDK4 and CDK2. |
Source : | E. coli |
Species : | Human |
Tag : | GST |
Form : | 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. |
Molecular Mass : | 84 kDa |
Purity : | > 85% |
Applications : | Western Blot |
Stability : | 1 year at -70 centigrade from the date of shipment. |
Storage : | Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. |
Concentration : | 0.1μg/μl |
Gene Name : | SMAD3 SMAD family member 3 [ Homo sapiens ] |
Official Symbol : | SMAD3 |
Synonyms : | SMAD3; SMAD family member 3; MAD, mothers against decapentaplegic homolog 3 (Drosophila) , MADH3, SMAD, mothers against DPP homolog 3 (Drosophila); mothers against decapentaplegic homolog 3; HsT17436; JV15 2; mad3; hMAD-3; hSMAD3; MAD homolog 3; mad homolog JV15-2; mad protein homolog; mothers against DPP homolog 3; SMA- and MAD-related protein 3; SMAD, mothers against DPP homolog 3; MAD, mothers against decapentaplegic homolog 3; LDS1C; MADH3; JV15-2; HSPC193; MGC60396; DKFZp586N0721; DKFZp686J10186; |
Gene ID : | 4088 |
mRNA Refseq : | NM_005902 |
Protein Refseq : | NP_005893 |
MIM : | 603109 |
UniProt ID : | P84022 |
Chromosome Location : | 15q21-q22 |
Pathway : | Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Adipogenesis, organism-specific biosystem; Alpha6-Beta4 Integrin Signaling Pathway, organism-specific biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; Cell cycle, organism-specific biosystem; Cell cycle, organism-specific biosystem; |
Function : | R-SMAD binding; RNA polymerase II activating transcription factor binding; beta-catenin binding; chromatin DNA binding; co-SMAD binding; collagen binding; core promoter proximal region sequence-specific DNA binding; double-stranded DNA binding; metal ion binding; phosphatase binding; protein binding; contributes_to protein binding transcription factor activity; protein homodimerization activity |
Products Types
◆ Recombinant Protein | ||
SMAD3-4147R | Recombinant Rhesus Macaque SMAD3 Protein, His (Fc)-Avi-tagged | +Inquiry |
SMAD3-304H | Recombinant Human SMAD3 Protein, His-tagged | +Inquiry |
Smad3-305M | Recombinant Mouse Smad3 Protein, His-tagged | +Inquiry |
Smad3-5951M | Recombinant Mouse Smad3 Protein, Myc/DDK-tagged | +Inquiry |
SMAD3-2041H | Recombinant Human SMAD3 Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Lysates | ||
SMAD3-001MCL | Recombinant Mouse SMAD3 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
0
Inquiry Basket