Active Recombinant Human SPHK1 protein, GST-tagged
Cat.No. : | SPHK1-174H |
Product Overview : | Recombinant Human SPHK1 fused with an N-terminal GST tag was expressed in Insect cells. |
- Specification
- Gene Information
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Description : | SPHK1 catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (SPP) which is a novel lipid messenger with both intracellular and extracellular functions. Various stimuli increase cellular levels of SPP by activation of SPHK1. Competitive inhibitors of SPHK1 block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors. SPHK1/sphingosine-1-phosphate pathway contributes to colon carcinogenesis. SPHK1 plays a key role in the generation and release of proinflammatory mediators triggered by anaphylatoxins from human macrophages and also is involved in the process of immune cell motility. |
Source : | Insect cells |
Species : | Human |
Tag : | GST |
Form : | 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. |
Bio-activity : | 500 nmol/min/mg |
Molecular Mass : | ~71 kDa |
Purity : | >70% |
Applications : | Kinase Assay |
Stability : | 1 year at -70 centigrade from the date of shipment |
Storage : | Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. |
Concentration : | 0.1μg/μl |
Gene Name : | SPHK1 sphingosine kinase 1 [ Homo sapiens ] |
Official Symbol : | SPHK1 |
Synonyms : | SPHK1; sphingosine kinase 1; SPHK; SK 1; SPK 1; |
Gene ID : | 8877 |
mRNA Refseq : | NM_001142601 |
Protein Refseq : | NP_001136073 |
MIM : | 603730 |
UniProt ID : | Q9NYA1 |
Chromosome Location : | 17q25.2 |
Pathway : | Association of TriC/CCT with target proteins during biosynthesis, organism-specific biosystem; Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Chaperonin-mediated protein folding, organism-specific biosystem; Fc gamma R-mediated phagocytosis, organism-specific biosystem; Fc gamma R-mediated phagocytosis, conserved biosystem; Fc-epsilon receptor I signaling in mast cells, organism-specific biosystem; |
Function : | ATP binding; D-erythro-sphingosine kinase activity; DNA binding; calmodulin binding; diacylglycerol kinase activity; magnesium ion binding; magnesium ion binding; nucleotide binding; protein binding; protein phosphatase 2A binding; sphinganine kinase activity; transferase activity; |
Products Types
◆ Recombinant Protein | ||
SPHK1-091H | Recombinant Human SPHK1 Protein, His-tagged | +Inquiry |
SPHK1-0269H | Recombinant Human SPHK1 Protein (M1-S363), Tag Free | +Inquiry |
SPHK1-12H | Recombinant Human SPHK1 Protein, N-His-tagged | +Inquiry |
SPHK1-012H | Recombinant Human SPHK1 Protein, His-tagged | +Inquiry |
SPHK1-4130H | Recombinant Human SPHK1 Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Lysates | ||
SPHK1-001HCL | Recombinant Human SPHK1 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionElevated SPHK1 levels have been associated with drug resistance in cancer cells, making it a potential target to overcome resistance mechanisms.
SPHK1 regulates immune cell trafficking and function by modulating S1P levels. This is significant in autoimmune diseases and inflammatory responses.
Understanding the role of SPHK1 in different diseases may contribute to the development of personalized therapeutic approaches, tailoring treatments based on individual patient characteristics and disease profiles.
SPHK1 is involved in endothelial cell functions and angiogenesis, making it a potential target in the treatment of vascular diseases.
Yes, combining SPHK1 inhibitors with other therapeutic agents is being explored to enhance treatment efficacy, especially in cancer.
Customer Reviews (3)
Write a reviewIts seamless integration makes it possible to explore different research avenues, expanding the scope and impact of my investigations.
With the manufacturer's excellent technical support and its wide range of applications, the SPHK1 protein has become an indispensable tool, aiding in my research endeavors and driving meaningful scientific discoveries.
Their team of experts is readily available to address any issues that may arise, providing solutions to problems and guiding me through the experimental process.
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