||Recombinant Human SV2C (454-579 aa) protein fused with GST tag was expressed in E. coli.
||This region of the protein is known as the luminal domain loop, between transmembrane domains 7 and 8, and has been shown to be the location of botulinum neurotoxin type A (BoNT/A) binding. Three posters demonstrating the functionality of the GST-SV2c fusion protein as a receptor for BoNT/A are available on this website (poster tab). Recent data from our laboratory indicate improved specificity and reliable detection of as little as 1.25 pg BoNT/A when captured by the SV2c receptor. A full description of the optimized parameters will be available soon.
||Each vial, when reconsitituted with 100 ul of water contains 100 ul of GST-SV2C in 20 Mm HEPES, pH 7.4, 1.25% lactose. Handle the product gently. Do not votex.
||This product is supplied as a lyophilized powder which has been stoppered under vacuum. Store lyophilized vials at 2-8 centigrade. Once dissolved, aliquot and store the product at -20 centigrade. Refrain from multiple freeze/thaw cycles.
||Good laboratory technique should be employed in the safe handling of this product. Wear appropriate laboratory attire including a lab coat, gloves and safety glasses. Nitrile gloves are recommended when handling lyophilized material. This product is intended for research purposes by qualified personnel only. It is not intended for use in humans or as a diagnostic agent.