| Description : |
TIGIT, also known as V-set and transmembrane domain-containing protein 3 (VSTM3) or V-set and immunoglobulin domain-containing protein 9 (VSIG9) is a new surface protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed on regulatory, memory, activated T cells and NK cells. It binds PVR with high affinity, and PVRL2 with lower affinity, but not PVRL3. Knockdown of TIGIT with siRNA in human memory T cells did not affect T cell responses, however, TIGIT inhibits NK cytotoxicity directly through its ITIM. TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells. The binding of PVR to TIGIT on human dendritic cells enhanced the production of IL-10 and diminished the production of IL-12p40. In addition, TIGIT counter inhibits the NK-mediated killing of tumor cells and protects normal cells from NK-mediated cytotoxicity thus providing an "alternative self" mechanism for MHC class I inhibition. |
| Source : |
CHO |
| Species : |
Human |
| Tag : |
Fc |
| Predicted N Terminal : |
Met 22 |
| Form : |
Lyophilized from sterile PBS, pH 7.4. |
| Bio-activity : |
Measured by its ability to bind with human CD155-Fc in a functional ELISA. Measured by its ability to bind with mouse PVRFch in a functional ELISA. |
| Molecular Mass : |
The recombinant mature human TIGIT protein consists of 349 amino acids and has a calculated molecular mass of 38.8 KD. |
| Endotoxin : |
<1.0EU/ug, LAL method |
| Purity : |
>95% (SDS-PAGE) |
| Stability : |
Samples are stable for up to twelve months from date of receipt at -70ºC. |
| Storage : |
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
