Active Recombinant Human TNF protein, hFc-tagged
Cat.No. : | TNF-234H |
Product Overview : | Recombinant Human TNF protein(NP_000585.2)(Val77-Leu233), fused with hFc tag, was expressed in HEK293. |
- Specification
- Gene Information
- Related Products
Source : | HEK293 |
Species : | Human |
Tag : | hFc |
Form : | Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA. |
Bio-activity : | Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 1-5 ng/mL. |
Molecular Mass : | The recombinant human TNF consists of 417 amino acids and predicts a molecular mass of 45.8 kDa. |
Proteinlength : | Val77-Leu233 |
Endotoxin : | < 1.0 EU per μg protein as determined by the LAL method. |
Purity : | > 90 % as determined by SDS-PAGE. |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name : | TNF tumor necrosis factor [ Homo sapiens ] |
Official Symbol : | TNF |
Synonyms : | TNF; tumor necrosis factor; TNFA, tumor necrosis factor (TNF superfamily, member 2); DIF; TNF superfamily; member 2; TNF alpha; TNFSF2; TNF-a; cachectin; APC1 protein; TNF, monocyte-derived; TNF, macrophage-derived; TNF superfamily, member 2; tumor necrosis factor alpha; tumor necrosis factor-alpha; tumor necrosis factor ligand superfamily member 2; TNFA; TNF-alpha; |
Gene ID : | 7124 |
mRNA Refseq : | NM_000594 |
Protein Refseq : | NP_000585 |
MIM : | 191160 |
UniProt ID : | P01375 |
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◆ Lysates | ||
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Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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