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Active Recombinant Human TNF protein, His-Avi-tagged, Biotinylated

Cat.No. : TNF-361H
Product Overview : Biotinylated Recombinant Human TNF protein(Val77-Leu233) is expressed from HEK293 with His tag and Avi tag at the C-terminal.
  • Specification
  • Gene Information
  • Related Products
Source : HEK293
Species : Human
Tag : His-Avi
Form : Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Bio-activity : Immobilized Human TNF R2, mFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human TNF alpha, His Tag with the EC50 of 3.5ng/ml determined by ELISA. See testing image for detail.
Molecular Mass : The protein has a predicted MW of 20.3 kDa. Due to glycosylation, the protein migrates to 21-24 kDa based on Tris-Bis PAGE result.
Protein Length : Val77-Leu233
Endotoxin : Less than 1EU per ug by the LAL method.
Purity : > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Storage : Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Reconstitution : Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Gene Name : TNF tumor necrosis factor [ Homo sapiens ]
Official Symbol : TNF
Synonyms : TNF; tumor necrosis factor; TNFA, tumor necrosis factor (TNF superfamily, member 2); DIF; TNF superfamily; member 2; TNF alpha; TNFSF2; TNF-a; cachectin; APC1 protein; TNF, monocyte-derived; TNF, macrophage-derived; TNF superfamily, member 2; tumor necrosis factor alpha; tumor necrosis factor-alpha; tumor necrosis factor ligand superfamily member 2; TNFA; TNF-alpha
Gene ID : 7124
mRNA Refseq : NM_000594
Protein Refseq : NP_000585
MIM : 191160
UniProt ID : P01375

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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