Active Recombinant Human TNFSF13B protein, hFc-tagged

• Cat.No.: TNFSF13B-004H
• Product Overview: Recombinant Human TNFSF13B protein(Q9Y275-1)(Ala134-Leu285), fused with hFc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: hFc
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA. Immobilized recombinant human BAFF at 1 μg/mL (100 μl/well) can bind human TNFRSF17. The EC50 of human TNFRSF17 is 0.07 μg/mL.2. Measured in a cell proliferation assay in mouse splenocytes. The ED50 for this effect is typically 0.6-3.2 ng/mL.
• Molecular Mass: The recombinant human BAFF/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 389 amino acids and has a predicted molecular mass of 43.7 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhBAFF/Fc monomer is approximately 48-55 kDa due to glycosylation.
• Proteinlength: Ala134-Leu285
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: TNFSF13B tumor necrosis factor (ligand) superfamily, member 13b [ Homo sapiens ]
• Official Symbol: TNFSF13B
• Synonyms: TNFSF13B; tumor necrosis factor (ligand) superfamily, member 13b; TNFSF20; tumor necrosis factor ligand superfamily member 13B; BAFF; BLYS; CD257; TALL 1; TALL1; THANK; delta BAFF; Delta4 BAFF; B-lymphocyte stimulator; B-cell-activating factor; ApoL related ligand TALL-1; TNF homolog that activates apoptosis; dendritic cell-derived TNF-like molecule; tumor necrosis factor-like protein ZTNF4; TNF and ApoL-related leukocyte expressed ligand 1; tumor necrosis factor (ligand) superfamily, member 20; DTL; ZTNF4; TALL-1
• Gene ID: 10673
• mRNA Refseq: NM_001145645
• Protein Refseq: NP_001139117
• MIM: 603969
• UniProt ID: Q9Y275