Active Recombinant Human VTN/TMSB4X protein

• Cat.No.: VTN-95H
• Product Overview: Recombinant human vitronectin (62–398aa) domain and full-length human Thymosin-b4 fusion protein cDNA were expressed in E. coli

Specification

• Source: E. coli
• Species: Human
• Form: 1.0 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, arginine, DTT and Glycerol.
• Bio-activity: When coated onto tissue culture plastic, VTB fusion protein promotes one half maximal attachment of human cardiomytes cells in serum-free medium at< 0.1 µg / cm2. Maximum attachment should occur at approximately 0.5 µg / cm2.
• AA Sequence: MTRGDVFTMPEDEYTVYDDGEEKNN ATVHEQVGGPSLTSDLQAQSKGNPE QTPVLKPEEEAPAPEVGASKPEGID SRPETLHPGRPQPPAEEELCSGKPF DAFTDLKNGSLFAFRGQYCYELDEK AVRPGYPKLIRDVWGIEGPIDAAFT RINCQGKTYLFKGSQYWRFEDGVLD PDYPRNISDGFDGIPDNVDAALALP AHSYSGRERVYFFKGKQYWEYQFQH QPSQEECEGSSLSAVFEHFAMMQRD SWEDIFELLFWGRTSAGTRQPQFIS RDWHGVPGQVDAAMAGRIYISGMAP RPSLAKKQRFRHRNRKGYRSQRGHS RGRNQNSRRPSRGGGGSGGGGSNIE FSDKPDMAEIEKFDKSKLKKTETQE KNPLPSKETIEQEKQAGES
• Endotoxin: < 30 EU per 1 µg of the protein by the LAL method
• Purity: >95% by SDS-PAGE
• Applications: 1. When coated at 0.5-1 ug/ ml per cm2 and combined with cardiomyocytes culture medium, this recombinant protein can be used as matrix protein for benefiting cardiomyocytes differentiation in vitro.
• Storage: Keep at -20centigrade for long term storage. Product is stable at 4 centigrade for at least 7 days.

Gene Information

• Gene Name: VTN vitronectin [ Homo sapiens ]
• Official Symbol: VTN
• Synonyms: VTN; vitronectin; vitronectin (serum spreading factor, somatomedin B, complement S protein); complement S protein; serum spreading factor; somatomedin B; VN; epibolin; S-protein; complement S-protein; serum-spreading factor; V75; VNT
• Gene ID: 7448
• mRNA Refseq: NM_000638
• Protein Refseq: NP_000629
• MIM: 193190
• UniProt ID: P04004
• Chromosome Location: 17q11.2
• Pathway: ECM-receptor interaction, organism-specific biosystem; ECM-receptor interaction, conserved biosystem; FOXA1 transcription factor network, organism-specific biosystem; Focal Adhesion, organism-specific biosystem; Focal adhesion, organism-specific biosystem
• Function: extracellular matrix binding; heparin binding; integrin binding; polysaccharide binding; scavenger receptor activity