Active Recombinant Human WNT3A protein

• Cat.No.: WNT3A-523H
• Product Overview: Recombinant Human WNT3A(Ser19-Lys352) was expressed in CHO.

Specification

• Description: Protein Wnt-3a is a protein that is encoded by the WNT3A gene. The WNT gene family consists of structurally related genes that encodesecreted signaling proteins. These proteins have been implicated in oncogenesis, adipogenesis, etc. and in several other developmentalprocesses, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family.Human Wnt3a shows 96% amino acid identity to mouse Wnt3a protein.
• Source: CHO
• Species: Human
• Form: Tris-HCl buffer pH 7.2-7.4, 0.5% CHAPS, 0.1% BSA.
• Bio-activity: Wnt3a activity has been measured using TCF-based Wnt reporter stable cell lines (HEK293 and NIH3T3). 4 ng/mL of Wnt3a generatefourteen- to twenty-fold increase of luciferase activity compared to control (buffer without Wnt3a).
• Molecular Mass: Predicted M.W.: 37 kDa;Actual M.W.: 41 kDa evaluated by SDS-PAGE under reducing conditions
• Purity: >75% evaluated by SDS-PAGE under reducing conditions
• Storage: This preparation can be stored at 4 centigrade for 6 months; Storage under frozen conditions may decrease activity; avoid freeze-thaw.
• Concentration: 4 µg/mL

Gene Information

• Gene Name: WNT3A wingless-type MMTV integration site family, member 3A [ Homo sapiens ]
• Official Symbol: WNT3A
• Synonyms: WNT3A; wingless-type MMTV integration site family, member 3A; protein Wnt-3a; MGC119418; MGC119419; MGC119420
• Gene ID: 89780
• mRNA Refseq: NM_033131
• Protein Refseq: NP_149122
• MIM: 606359
• UniProt ID: P56704
• Chromosome Location: 1q42
• Pathway: Basal cell carcinoma, organism-specific biosystem; Basal cell carcinoma, conserved biosystem; Canonical Wnt signaling pathway, organism-specific biosystem; Class B/2 (Secretin family receptors), organism-specific biosystem; DNA damage response (only ATM dependent), organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; HTLV-I infection, organism-specific biosystem
• Function: extracellular matrix structural constituent; frizzled-2 binding; protein binding; protein domain specific binding; receptor agonist activity; transcription coactivator activity