Active Recombinant MERS-CoV (NCoV / Novel coronavirus) S Protein, His-tagged
Cat.No. : | S-598V |
Product Overview : | Recombinant extracellular domain of spike protein (Human betacoronavirus 2c EMC/2012)( AFS88936.1) (Met1-Trp1297) was fused with a polyhistidine tag at the C-terminus. |
- Specification
- Gene Information
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Source : | Baculovirus-Insect Cells |
Species : | MERS-CoV |
Tag : | His |
Predicted N Terminal : | Tyr 18 |
Form : | Lyophilized from sterile 20mM Tris, 500mM Nacl, pH 7.4, 10% glycerol, 5%~8% trehalose and mannitol. |
Bio-activity : | Measured by its binding ability in a functional ELISA. 1. Immobilized Spike (HCoV-EMC/2012) (ECD, aa 1-1297) at 10 μg/ml (100 μl/well) can bind biotinylated human ACE2-Fc. The EC50 of biotinylated human ACE2-Fc is 0.9-1.4 μg/ml. 2. Immobilized Spike (HCoV |
Molecular Mass : | The recombinant extracellular domain of spike protein (Human betacoronavirus 2c EMC/2012) comprises 1289 amino acids and has a predicted molecular mass of 142.2 kDa. It migrates as an approximately 157 kDa band in SDS-PAGE under reducing conditions. |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method. |
Purity : | >85 % as determined by SDS-PAGE. |
Stability : | Samples are stable for up to twelve months from date of receipt at -70ºC. |
Storage : | Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
Gene Name : | S S protein [ Middle East respiratory syndrome coronavirus ] |
Official Symbol : | S |
Synonyms : | spike glycoprotein; S protein |
Gene ID : | 14254594 |
mRNA Refseq : | |
Protein Refseq : | |
MIM : | |
UniProt ID : |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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