Description : |
This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer, which plays a role in bone and cartilage development. While a homozygous knockout mouse for this gene exhibits embryonic lethality, conditional knockout mice have defects in bone, cartilage and heart development. |
Source : |
E. coli |
Species : |
Mouse |
Tag : |
His |
Form : |
Freeze-dried powder |
Molecular Mass : |
15kDa as determined by SDS-PAGE reducing conditions |
Protein length : |
Gln281~Arg394 |
Endotoxin : |
<1.0EU per 1µg (determined by the LAL method) |
Purity : |
> 97% |
Characteristic : |
The isoelectric point is 8.2. |
Applications : |
Positive Control; Immunogen; SDS-PAGE; WB. |
Stability : |
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition. |
Storage : |
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months. |
Concentration : |
200µg/mL |
Storage buffer : |
10mM PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and Proclin300. |
Reconstitution : |
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex |