Active Recombinant Mouse Car2 Protein, His-tagged

• Cat.No.: Car2-886M
• Product Overview: Recombinant mouse CA2 (AAH55291.1) (Met 1-Lys 260) was expressed, with a C-terminal polyhistidine tag.

Specification

• Source: E. coli
• Species: Mouse
• Tag: His
• Predicted N Terminal: Ser 2
• Form: Lyophilized from sterile 50mM Tris, 150mM NaCl, pH 7.51, 5%~8% trehalose and mannitol.
• Bio-activity: Measured by its esterase activity. The specific activity is >100 pmoles/min/μg.
• Molecular Mass: The recombinant mouse CA2 comprises 270 amino acids and has a calculated molecular mass of 30.4 kDa. The recombinant protein migrates as an approximately 30-33 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >96 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: Car2 carbonic anhydrase 2 [ Mus musculus ]
• Official Symbol: Car2
• Synonyms: Ca2; CAII; Car-2; Ltw-5; Lvtw-5; AI131712; CA II; CA-II; carbonate dehydratase II; carbonic anhydrase II
• Gene ID: 12349
• mRNA Refseq: NM_009801
• Protein Refseq: NP_033931
• UniProt ID: P00920
• Chromosome Location: 3 A1; 3 3.23 cM
• Pathway: Collecting duct acid secretion, organism-specific biosystem; Nitrogen metabolism, organism-specific biosystem; Pancreatic secretion, conserved biosystem
• Function: arylesterase activity; carbonate dehydratase activity; metal ion binding