Recombinant Mouse Casp8 protein, His & T7-tagged

• Cat.No.: Casp8-7830M
• Product Overview: Recombinant Mouse Casp8 aa. (Ser219~Gly376 (Accession # O89110)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: This gene is part of a family of caspases, aspartate-specific cysteine proteases well studied for their involvement in immune and apoptosis signaling. This protein, an initiator of apoptotic cell death, is activated by death-inducing tumor necrosis family receptors and targets downstream effectors. In mouse deficiency of this gene can cause embryonic lethality. This protein may have a role in embryogenesis. Alternative splicing results in multiple transcript variants that encode different protein isoforms.
• Source: E. coli
• Species: Mouse
• Tag: His & T7
• Form: Freeze-dried powder
• Molecular Mass: 21 kDa as determined by SDS-PAGE reducing conditions
• Protein length: Ser219~Gly376 (Accession # O89110)
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 7.2.
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as liquid form in Phosphate buffered saline(PBS), pH 7.4.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: Casp8 caspase 8 [ Mus musculus (house mouse) ]
• Official Symbol: Casp8
• Synonyms: Casp8; caspase 8; MACH; Mch5; FLICE; CASP-8; caspase-8; Fas-linked ICE-like protease
• Gene ID: 12370
• mRNA Refseq: NM_001080126.1
• Protein Refseq: NP_001073595.1
• UniProt ID: O89110