Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Active Recombinant Mouse CNTF Protein, His-tagged

Cat.No. : CNTF-995M
Product Overview : Recombinant mouse CNTF (NP_740756.1) (Ala2-Met198) was expressed with a polyhistidine tag at the N-terminus.
  • Specification
  • Gene Information
  • Related Products
Source : E. coli
Species : Mouse
Tag : His
Predicted N Terminal : His
Form : Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Bio-activity : 1. Measured by its binding ability in a functional ELISA. Immobilized mouse CNTF at 10 μg/ml (100 μl/well) can bind biotinylated rat CNTFR-His. The EC50 of biotinylated rat CNTFR-His is 5-12 ng/ml.2. Measured in a cell proliferation assay using TF-1 human
Molecular Mass : The recombinant mouse CNTF consists of 215 amino acids and predicts a molecular mass of 24.7 KDa. It migrates as an approximately 25 KDa band in SDS-PAGE under reducing conditions.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method.
Purity : >95 % as determined by SDS-PAGE.
Stability : Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage : Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
Gene Name : Cntf ciliary neurotrophic factor [ Mus musculus ]
Official Symbol : CNTF
Synonyms : CNTF; ciliary neurotrophic factor; AI429687; MGC41235;
Gene ID : 12803
mRNA Refseq : NM_170786
Protein Refseq : NP_740756
MIM :
UniProt ID :
Pathway : Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Delta-Notch Signaling Pathway, organism-specific biosystem; Jak-STAT signaling pathway, organism-specific biosystem; Jak-STAT
Function : ciliary neurotrophic factor receptor binding; cytokine activity; growth factor activity; interleukin-6 receptor binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (7)

Ask a question
How does CNTF affect glial cell function? 08/18/2021

CNTF plays a crucial role in regulating glial cell function. It can modulate astrocyte and oligodendrocyte differentiation, promote myelination, and regulate the release of neurotrophic factors from glial cells. CNTF also influences glial cell-mediated immune responses and their interaction with neurons in various pathological conditions.

What are the signaling pathways activated by CNTF in neurons and glial cells? 11/28/2020

CNTF activates multiple signaling pathways, including JAK/STAT, PI3K/AKT, and MAPK/ERK, in both neurons and glial cells. These pathways play crucial roles in regulating gene expression, cell survival, differentiation, and synaptic plasticity.

What is the role of CNTF in neuroinflammation? 09/08/2019

CNTF plays a significant role in modulating neuroinflammatory responses. It can regulate the activation and function of microglia and astrocytes, leading to the production of pro-inflammatory or anti-inflammatory factors. CNTF also influences the recruitment and activation of immune cells in the central nervous system.

How does CNTF affect synaptic plasticity? 10/22/2016

CNTF has been shown to modulate synaptic plasticity, which underlies learning and memory processes. It promotes the formation and stabilization of synapses, regulates neurotransmitter release, and influences synaptic strength and plasticity through its interaction with specific receptors and downstream signaling molecules.

What are the challenges in developing CNTF-based therapies? 10/16/2016

Developing CNTF-based therapies faces challenges such as the need for targeted delivery to specific brain regions, potential side effects due to systemic administration, and the need to optimize dosing and treatment regimens. Additionally, understanding the precise mechanisms of CNTF action and identifying suitable patient populations for treatment are important considerations.

How does CNTF promote neuronal survival and regeneration? 04/14/2016

CNTF exerts neuroprotective effects by promoting neuronal survival and regeneration through the activation of various intracellular signaling pathways. It enhances the expression of neurotrophic factors, regulates apoptosis-related genes, and stimulates axonal outgrowth and synaptic remodeling.

Can CNTF be a potential therapeutic approach for neurodegenerative diseases? 02/07/2016

CNTF shows promise as a potential therapeutic approach for neurodegenerative diseases, such as amyotrophic lateral sclerosis and retinal degeneration. Its neuroprotective and neurotrophic effects make it a potential candidate for promoting neuronal survival, preventing neurodegeneration, and improving functional recovery in these conditions.

Customer Reviews (3)

Write a review
Reviews
03/04/2020

    It is highly durable and meets the requirements for long-term experiments.

    06/02/2017

      A trusted aide, this reagent is instrumental on the path of scientific inquiry.

      09/01/2016

        Employing this protein reagent allows me to obtain precise structural information in protein interaction studies, offering critical insights for a deeper understanding of protein function.

        Ask a Question for All CNTF Products

        Required fields are marked with *

        My Review for All CNTF Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /

        Stay Updated on the Latest Bioscience Trends