Active Recombinant Mouse Eno1 Protein, His-tagged, Bioactivity Validated

• Cat.No.: Eno1-244M
• Product Overview: Recombinant Mouse Eno1(1-434aa) fused with His tag at N-terminal was expressed in E. coli. Bioactivity Validated.

Specification

• Description: Eno1 also known as Alpha-Enolase, is one of three enolase isoenzymes and a glycolytic enzyme expressed in most tissues. This protein plays a key role in anaerobic metabolism under hypoxic conditions and may act as a cell surface plasminogen receptor during tissue invasion. Abnormal expression of Eno1 is associated with tumor progression in some cases of breast and lung cancer. It also has been identified as an autoantigen associated with Hashimoto's encephalopathy and severe asthma.
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
• Bio-activity: Specific activity is > 6,000 pmol/min/ug, and is defined as the amount of enzyme that convert 1.0 pmole of 2-phosphoglycerate to phosphoenolpyruvate per minute at pH 6.5 at 37C in a couple system with PK and LDH.
• Molecular Mass: 49.5 kDa (457aa) confirmed by MALDI-TOF
• Protein length: 1-434aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSILRIH AREIFDSRGN PTVEVDLYTA KGLFRAAVPS GASTGIYEAL ELRDNDKTRF MGKGVSQAVE
  HINKTIAPAL VSKKVNVVEQ EKIDKLMIEM DGTENKSKFG ANAILGVSLA VCKAGAVEKG VPLYRHIADL AGNPEVILPV PAFNVINGGS
  HAGNKLAMQE FMILPVGASS FREAMRIGAE VYHNLKNVIK EKYGKDATNV GDEGGFAPNI LENKEALELL KTAIAKAGYT DQVVIGMDVA
  ASEFYRSGKY DLDFKSPDDP SRYITPDQLA DLYKSFVQNY PVVSIEDPFD QDDWGAWQKF TASAGIQVVG DDLTVTNPKR IAKAASEKSC
  NCLLLKVNQI GSVTESLQAC KLAQSNGWGV MVSHRSGETE DTFIADLVVG LCTGQIKTGA PCRSERLAKY NQILRIEEEL GSKAKFAGRS
  FRNPLAK
• Purity: > 90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1mg/ml (determined by Absorbance at 280nm)

Gene Information

• Gene Name: Eno1 enolase 1, alpha non-neuron [ Mus musculus ]
• Official Symbol: Eno1
• Synonyms: ENO1; enolase 1, alpha non-neuron; alpha-enolase; NNE; non-neural enolase; c-Myc promoter binding protein; 2-phospho-D-glycerate hydrolase; 2-phospho-D-glycerate hydro-lyase; Eno-1; MBP-1; AL022784; 0610008I15; MGC103111; MGC107267
• Gene ID: 13806
• mRNA Refseq: NM_023119
• Protein Refseq: NP_075608
• UniProt ID: P17182