Active Recombinant Mouse F7 Protein, His-tagged

• Cat.No.: F7-828M
• Product Overview: Recombinant mouse FⅦ (NP_034302.2) (Met 1-Leu 446) was fused with the a polyhistidine tag at the C-terminus.

Specification

• Source: CHO Stable Cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: Val 25 & Ala 42
• Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Bio-activity: Measured by its binding ability in a functional ELISA.Immobilized mouse F7-his at 10 μg/ml (100 μl/well) can bind biotinylated mouse F3-his. The EC50 of biotinylated mouse F3-his is 0.1-0.3 μg/ml.
• Molecular Mass: The mature form of mouse FⅦ consists of 416 amino acids after removal of the signal peptide and the propeptid, and has a predicted molecular mass of 47 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm FⅦ is approximately 56-63 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >90 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: F7 coagulation factor VII [ Mus musculus ]
• Official Symbol: F7
• Synonyms: F7; coagulation factor VII; serum prothrombin conversion accelerator; Cf7; FVII; mfVII; AI132620
• Gene ID: 14068
• mRNA Refseq: NM_010172
• Protein Refseq: NP_034302
• Pathway: Blood Clotting Cascade, organism-specific biosystem; Complement and Coagulation Cascades, organism-specific biosystem; Complement and coagulation cascades, organism-specific biosystem; Complement and coagulation cascades, conserved biosystem; Extrinsic Pa
• Function: calcium ion binding; catalytic activity; endopeptidase activity; glycoprotein binding; hydrolase activity; peptidase activity; receptor binding; serine-type endopeptidase activity; serine-type peptidase activity