Active Recombinant Mouse Gpt protein, His-tagged

• Cat.No.: Gpt-131M
• Product Overview: Recombinant Mouse Gpt(1-496 aa) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Description: Gpt, also known as alanine aminotransferases 1, catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. This protein plays a key role in the intermediary metabolism of glucose and amino acids. It is widely used as an index of liver integrity or hepatocellular damage in clinical settings.
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Liquid. In Phosphate Buffered Saline (pH7.4) containing 1mM DTT, 10% glycerol
• Bio-activity: Specific activity is > 40units/mg, and is defined as the amount of
  enzyme that convert 1umole of L-Alanine to L-Glutamate per minute at pH 7.5 at 37°C
• Molecular Mass: 57.5 kDa (519 aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMASQRND RIQASRNGLK GKVLTLDTMN PCVRRVEYAV RGPIVQRALE LEQELRQGVK KPFTEVIRAN
  IGDAQAMGQR PITFFRQVLA LCVYPNLLSS PDFPEDAKRR AERILQACGG HSLGAYSISS GIQPIREDVA QYIERRDGGI PADPNNIFLS
  TGASDAIVTM LKLLVAGEGR ARTGVLIPIP QYPLYSAALA ELDAVQVDYY LDEERAWALD IAELRRALCQ ARDRCCPRVL CVINPGNPTG
  QVQTRECIEA VIRFAFEEGL FLMADEVYQD NVYAEGSQFH SFKKVLTEMG PPYATQQELA SFHSVSKGYM GECGFRGGYV EVVNMDAEVQ
  KQMAKLMSVR LCPPVPGQAL MGMVVSPPTP SEPSFKQFQA ERQEVLAELA AKAKLTEQVF NEAPGIRCNP VQGAMYSFPQ IQLPLKAVQR
  AQDLGLAPDM FFCLCLLEET GICVVPGSGF GQQEGTYHFR MTILPPMEKL RVLLEKLRHF HAKFTHEYS
• Purity: > 90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Absorbance at 280nm )

Gene Information

• Gene Name: Gpt glutamic pyruvic transaminase, soluble [ Mus musculus ]
• Official Symbol: Gpt
• Synonyms: GPT; glutamic pyruvic transaminase, soluble; alanine aminotransferase 1; ALT1; GPT 1; glutamic--alanine transaminase 1; glutamic--pyruvic transaminase 1; glutamate pyruvate transaminase 1; glutamic pyruvic transaminase 1, soluble; ALT; Gpt1; Gpt-1; 1300007J06Rik; 2310022B03Rik
• Gene ID: 76282
• mRNA Refseq: NM_182805
• Protein Refseq: NP_877957
• UniProt ID: Q8QZR5
• Chromosome Location: 15 D3; 15 36.28 cM
• Pathway: Alanine and aspartate metabolism, organism-specific biosystem; Alanine, aspartate and glutamate metabolism, organism-specific biosystem; Alanine, aspartate and glutamate metabolism, conserved biosystem; Amino acid synthesis and interconversion (transamination), organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of amino acids and derivatives, organism-specific biosystem
• Function: L-alanine:2-oxoglutarate aminotransferase activity; catalytic activity; pyridoxal phosphate binding; transaminase activity; transferase activity; transferase activity, transferring nitrogenous groups