Recombinant Mouse Gzmk protein(Gln44~Val227), His-tagged
Cat.No. : | Gzmk-5812M |
Product Overview : | Recombinant Mouse Gzmk protein(Gln44~Val227), fused with N-terminal His Tag, was expressed in E.coli. |
- Specification
- Gene Information
- Related Products
Source : | E.coli |
Species : | Mouse |
Tag : | N-His |
Protein length : | Gln44~Val227 |
Form : | PBS, pH7.4, containing 0.01% SKL, 5%Trehalose. |
Bio-activity : | Granzyme K is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Mouse granzyme K is synthesized as a precursor (263 residues) with a signal peptide (residues 1- 21), a propeptide (residues 22-25) and a mature chain (residues 26-263). The activity of recombinant mouse Granzyme K was measured by its ability to cleaves a thioester substrate Z-Lys-SBzl • HCl. The reaction was performed in 0.05 M Tris, 0.15 M NaCl, 0.01% Triton X-100, pH 8.0 (assay buffer), initiated by addition 50 μ L of various concentrations of GZMK(diluted by assay buffer) to 50 μL of 1.2 mM substrate and DTNB mixture. The final well serves as a negative control with no GZMK, replace with 50 μL assay buffer. Incubated at 25°C for 5min, then read at a wavelength of 405 nm. |
Molecular Mass : | 24.1kDa |
Endotoxin : | <1.0EU per 1μg (determined by the LAL method). |
Purity : | > 90% |
Storage : | Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months. |
Reconstitution : | Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex. |
Gene Name : | Gzmk granzyme K [ Mus musculus ] |
Official Symbol : | Gzmk |
Synonyms : | GZMK; granzyme K; |
Gene ID : | 14945 |
mRNA Refseq : | NM_008196 |
Protein Refseq : | NP_032222 |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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