Active Recombinant Mouse Igfbp2 protein(Met1-Gln305), hFc-tagged

• Cat.No.: Igfbp2-969M
• Product Overview: Recombinant Mouse Igfbp2 (NP_032368.2) (Met1-Gln305) was expressed in HEK293 with the Fc region of Human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Mouse
• Tag: C-hFc
• Protein length: Met1-Gln305
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to inhibit the biological activity of IGFII on MCF7 human breast adenocarcinoma cells. The ED50 for this effect is typically 0.05-0.4 μg/mL in the presence of 14 ng/mL human IGFII.
• Molecular Mass: The recombinant mouse Igfbp2 consists 512 amino acids and predicts a molecular mass of 56.6 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Igfbp2 insulin-like growth factor binding protein 2 [ Mus musculus ]
• Official Symbol: Igfbp2
• Synonyms: IGFBP2; insulin-like growth factor binding protein 2; insulin-like growth factor-binding protein 2; IGF-binding protein 2; IBP-2; Igfbp-2; AI255832; mIGFBP-2
• Gene ID: 16008
• mRNA Refseq: NM_008342
• Protein Refseq: NP_032368