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Active Recombinant Mouse IL12A & IL12B Protein, Fc & His-tagged

Cat.No. : IL12A & IL12B-1244M
Product Overview : Recombinant p35 subunit of mouse IL12, termed as IL12A (P43431) (Met 1-Ala 215) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; Recombinant p40 subunit of mouse IL12, termed as IL12B (P43432) (Met 1-Ser 335) was fused with
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  • Gene Information
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Source : HEK293
Species : Mouse
Tag : Fc & His
Predicted N Terminal : Arg 23 & Met 23
Form : Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Bio-activity : 1. Measured by its ability to mouse IL12RB2-His in a functional ELISA. 2. Measured in a cell proliferation assay using Anti-CD3-stimulated PBMC. The ED50 for this effect is typically 0.2-1 ng/mL.
Molecular Mass : The recombinant mouse IL12 heterodimer of IL12A/IL12B, IL12B is Fc chimera, comprises 758 (204 + 554) amino acids and has a calculated molecular mass of 85.9 (23.1 + 62.8) kDa. The apparent molecular mass of rh IL12 heterodimer is approximately 28 & 75 kDa respectively in SDS-PAGE under reducing conditions.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method.
Purity : >85 % as determined by SDS-PAGE.
Stability : Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage : Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
Gene Name : Il12a interleukin 12a [ Mus musculus ]
Official Symbol : IL12A
Synonyms : IL12A; interleukin 12a; interleukin-12 subunit alpha; CLMF p35; IL-12 subunit p35; interleukin 12a p35 subunit; cytotoxic lymphocyte maturation factor 35 kDa subunit; p35; Ll12a; Il-12a; IL-12p35; MGC151228; MGC151232;
Gene ID : 16159
mRNA Refseq : NM_001159424
Protein Refseq : NP_001152896
MIM :
UniProt ID :
Pathway : African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Amoebiasis, organism-specific biosystem; Amoebiasis, conserved
Function : contributes_to cytokine activity; cytokine activity; growth factor activity; interleukin-12 beta subunit binding; interleukin-12 receptor binding; interleukin-27 binding; protein heterodimerization activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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