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Recombinant Mouse Il12rb1 protein, His & GST-tagged

Cat.No. : Il12rb1-1734M
Product Overview : Recombinant Mouse Il12rb1 aa. (Gln197~Arg348 (Accession # Q60837)) fused with N-terminal His & GST tag was produced in E. coli cells.
  • Specification
  • Gene Information
  • Related Products
Source : E. coli
Species : Mouse
Tag : His & GST
Form : Freeze-dried powder
Molecular Mass : Predicted Molecular Mass: 46.5kDa
Protein length : Gln197~Arg348 (Accession # Q60837)
Endotoxin : <1.0EU per 1ug (determined by the LAL method)
Purity : >90%
Characteristic : The isoelectric point is 10.5.
Applications : SDS-PAGE; WB; ELISA; IP.
Stability : The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Storage buffer : Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Reconstitution : Reconstitute in sterile PBS, pH7.2-pH7.4.
Gene Name : Il12rb1 interleukin 12 receptor, beta 1 [ Mus musculus (house mouse) ]
Official Symbol : Il12rb1
Synonyms : Il12rb1; interleukin 12 receptor, beta 1; CD212; IL-12R[b]; interleukin-12 receptor subunit beta-1; IL-12 receptor beta component; IL-12 receptor subunit beta-1; IL-12R subunit beta-1; IL-12R-beta-1; Interleukin-12 receptor beta-1 chain precursor (IL-12R-beta1) (Interleukin-12 receptor beta) (IL-12 receptor beta component)
Gene ID : 16161
mRNA Refseq : NM_008353.2
Protein Refseq : NP_032379.2
UniProt ID : Q60837

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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Could you explain the significance of CRISPR/Cas9-mediated gene tagging in tracking IL12RB1 localization? 09/18/2021

Gene tagging allows real-time tracking of IL12RB1 subcellular localization.

Can you elaborate on the use of RNA-seq analysis to profile gene expression changes upon IL12RB1 activation? 07/02/2021

RNA-seq profiles gene expression changes upon IL12RB1 activation.

What are the applications of ChIP-seq in exploring IL12RB1's role in gene regulation? 01/23/2021

ChIP-seq investigates IL12RB1's impact on gene regulation networks.

What methods can be employed to assess IL12RB1 dimerization and its effect on downstream signaling? 06/13/2020

Crosslinking and immunoprecipitation assess IL12RB1 dimerization and signaling.

What techniques are suitable for studying IL12RB1's interaction with IL12A and IL23A? 02/14/2020

Co-immunoprecipitation and ELISA assay IL12RB1 interactions with cytokines.

How can the role of IL12RB1 in signal transduction be elucidated using knockdown experiments? 12/27/2019

Knockdown experiments reveal IL12RB1's contribution to signal transduction.

What are the advantages of using siRNA screening to identify IL12RB1-regulated pathways? 02/22/2019

siRNA screening identifies pathways influenced by IL12RB1 expression.

How do co-crystallization studies aid in understanding the molecular details of IL12RB1 interactions? 01/02/2019

Co-crystallization offers structural insights into IL12RB1 interactions.

How does flow cytometry provide insights into IL12RB1 expression levels on different cell types? 10/26/2018

Flow cytometry quantifies IL12RB1 levels on diverse cell populations.

What experimental approaches validate IL12RB1's involvement in cytokine-mediated immune responses? 06/25/2018

Functional assays validate IL12RB1's role in immune responses.

Customer Reviews (3)

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Reviews
04/06/2022

    Reliable in co-immunoprecipitation.

    10/18/2021

      Effective in asthma models.

      12/21/2017

        great in Western blot analysis.

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