| Product overview : |
Recombinant Mouse INS aa. (Phe25~Ser54 and Gly90~Asn110 linked by GGGGS) fused with His tag was produced in E. coli cells. |
| Source : |
E. coli |
| Species : |
Mouse |
| Tag : |
His |
| Form : |
Freeze-dried powder |
| Molecular Mass : |
Predicted Molecular Mass: 10.3kDa; Accurate Molecular Mass: 10kDa as determined by SDS-PAGE reducing conditions. |
| Protein length : |
Phe25~Ser54 and Gly90~Asn110 linked by GGGGS |
| Endotoxin : |
<1.0EU per 1ug (determined by the LAL method) |
| Purity : |
>98% |
| Characteristic : |
Isoelectric point is 7.1. |
| Applications : |
Cell culture; Activity Assays; In vivo assays. |
| Stability : |
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition. |
| Storage : |
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months. |
| Storage buffer : |
20mM Tris, 150mM NaCl,pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% Trehalose and Proclin300. |
| Reconstitution : |
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex |
