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Recombinant Mouse Lag3 protein, His-tagged

Cat.No.: Lag3-1831M
Product Overview: Mouse LAG-3 / CD223 Protein (Mouse LAG-3, His Tag) Gly 24 - Leu 442 (Accession # Q61790) was produced in human 293 cells (HEK293).
Description: Lymphocyte activation gene 3 protein (LAG3) is also known as CD antigen CD223 and protein FDC, which belongs to immunoglobulin (Ig) superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. LAG3 /CD223 involved in lymphocyte activation. LAG3 /CD223 binds to HLA class-II antigens.
Source: HEK293
Species: Mouse
Tag : His
Predicted N Terminal: Gly 24
Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass: Mouse LAG-3, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 47.1 kDa. The reducing (R) protein migrates as 55-65 kDa in SDS-PAGE due to glycosylation.
Protein length: AA Gly 24 - Leu 442
Endotoxin: Less than 1.0 EU per μg of the Mouse LAG-3, His Tag by the LAL method.
Purity: >95% as determined by SDS-PAGE.
Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: Lag3 lymphocyte-activation gene 3 [ Mus musculus (house mouse) ]
Official Symbol: Lag3
Synonyms: Ly66; CD223; LAG-3
Gene ID: 16768
mRNA Refseq: NM_008479.2
Protein Refseq: NP_032505.1
UniProt ID: Q61790

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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