Recombinant Mouse Nfkbib protein, His & T7-tagged

• Cat.No.: Nfkbib-1877M
• Product Overview: Recombinant Mouse Nfkbib aa. (Gly117~Pro345 (Accession # Q60778)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: This gene encodes an inhibitor of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kappaB). The encoded protein prevents NF-kappaB-mediated transcription activation by sequestering it in the cytosol. In response to signals that induce NF-kappaB, such as cytokines and growth factors, the encoded protein undergoes phosphorylation, triggering its rapid ubiquitination and proteasomal degradation. Alternative splicing results in multiple transcript variants.
• Source: E. coli
• Species: Mouse
• Tag: His & T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 28.5kDa.
• Protein length: Gly117~Pro345 (Accession # Q60778)
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 5.5.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: Nfkbib nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, beta [ Mus musculus (house mouse) ]
• Official Symbol: Nfkbib
• Synonyms: Scnn1a; sodium channel, nonvoltage-gated 1 alpha; ENaC; SCNEA; Scnn1; mENaC; amiloride-sensitive sodium channel subunit alpha; ENaC alpha; alpha-ENaC; alpha-NaCH; amiloride-sensitive epithelial sodium channel; epithelial Na(+) channel subunit alpha; epithelial sodium channel alpha subunit; nonvoltage-gated sodium channel 1 subunit alpha; sodium channel, nonvoltage-gated, type I, alpha polypeptide
• Gene ID: 18036
• mRNA Refseq: NM_001306222.1
• Protein Refseq: NP_001293151.1
• UniProt ID: Q60778