Recombinant Mouse SMAD2 Protein, GST & His-tagged

• Cat.No.: SMAD2-901M
• Product Overview: Recombinant mouse SMAD2 (Q62432-Isoform Long) (Ser2-Ser467) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Specification

• Source: Baculovirus-Insect Cells
• Species: Mouse
• Tag: GST & His
• Predicted N Terminal: Met
• Form: Lyophilized from sterile 20mM Tris, 500mM Nacl, pH 8.0, 10% glycerol, 3mM DTT1, 5%~8% trehalose and mannitol.
• Molecular Mass: The recombinant mouse SMAD2/GST chimera consists of 703 amino acids and has a calculated molecular mass of 80 kDa. The recombinant protein migrates as an approximately 90 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >90 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: Smad2 MAD homolog 2 (Drosophila) [ Mus musculus ]
• Official Symbol: SMAD2
• Synonyms: SMAD2; MAD homolog 2 (Drosophila); mothers against decapentaplegic homolog 2; Smad 2; SMAD family member 2; mad-related protein 2; mothers against DPP homolog 2; Madh2; Madr2; mMad2; Smad-2; 7120426M23Rik
• Gene ID: 17126
• mRNA Refseq: NM_001252481
• Protein Refseq: NP_001239410
• Pathway: Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Alpha6-Beta4 Integrin Signaling Pathway, organism-specific biosystem; Cell cycle, organism-specific biosystem; Cell cycle, conserved biosystem; Cell cycle signaling pa
• Function: I-SMAD binding; R-SMAD binding; SMAD binding; SMAD binding; activating transcription factor binding; chromatin binding; co-SMAD binding; double-stranded DNA binding; phosphatase binding; protein binding; sequence-specific DNA binding transcription factor