"Tnfsf4" Related Products

Expand
Expand

Recombinant Mouse Tnfsf4 protein, His-tagged

Cat.No.: Tnfsf4-21M
Product Overview: Recombinant Mouse Tnfsf4(Gln49-Leu198) fused with His tag at N-terminal was expressed in HEK293.
Description: OX40 Ligand (OX40L), also known as gp34, is a type II transmembrane glycoprotein belonging to the TNF superfamily. Murine OX40L cDNA encodes a 198 amino acid (aa) residue protein comprised of a 28 aa N-terminal cytoplasmic domain, a 20 aa transmembrane segment, and a 150 aa C-terminal extracellular domain. Human and murine OX40L share 46% sequence identity at the amino acid level. The OX40L is expressed on activated antigen presenting cells such as B cells, macrophages, dendritic cells, and on endothelial cells at the site of inflammation. The receptor for OX40L is OX40 (CD134) that is expressed predominantly on activated CD4+ T cells. Expression of OX40 is transient following engagement of T cell receptors. Ligation of OX40L by OX40 stimulates proliferation and differentiation of activated B cells, and increases immunoglobulin secretion. The expression of OX40L on B cells is up-regulated by CD40 ligation. Engagement of the OX40-OX40L system has co-stimulatory effects on T cells by stimulating the production of cytokines by T helper cells and increasing the survival of memory T cells. Blocking of the OX40-OX40L interaction in vitro inhibits co-stimulation resulting in decreased T cell proliferation and adhesion of T cells to endothelial cells. Inhibition of the OX40-OX40L interaction in disease models has beneficial effects in acute graft-versus-host disease, inflammatory bowel disease and decreases the development of collagen-induced arthritis and experimental leishmaniasis.
Source: HEK293
Species: Mouse
Tag : His
Predicted N Terminal: His
Form: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Bio-activity: Measured by its ability to co-stimulate IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED50 for this effect is typically 10-30 ng/mL in the presence of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody.
Molecular Mass: Predicted Molecular Mass: 18 kDa
SDS-PAGE: 21-25 kDa, reducing conditions
Protein length: Gln49-Leu198
Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution: Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Gene Name: Tnfsf4 tumor necrosis factor (ligand) superfamily, member 4 [ Mus musculus ]
Official Symbol: Tnfsf4
Synonyms: TNFSF4; tumor necrosis factor (ligand) superfamily, member 4; tumor necrosis factor ligand superfamily member 4; OX40 ligand; atherosclerosis 1; tax-transcriptionally activated glycoprotein 1 ligand; Ath1; gp34; Ath-1; Ox40l; TXGP1; CD134L; OX-40L; Txgp1l;
Gene ID: 22164
mRNA Refseq: NM_009452
Protein Refseq: NP_033478
UniProt ID: P43488

Online Inquiry

Note: There will be extra charge for optional service!
Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information


Price does not include shipping and packaging costs.Request quote for final price.

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.