Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Recombinant Rat Hsd11b2 protein, His-tagged

Cat.No. : Hsd11b2-20R
Product Overview : Recombinant Rat Hsd11b2(Leu58~Pro393) fused with Hhis tag at N-terminal was expressed in E. coli.
  • Specification
  • Gene Information
  • Related Products
Description : Hsd11b2 played an important role in many functions.
Source : E. coli
Species : Rat
Tag : His
Form : PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Protein length : Leu58~Pro393
Endotoxin : <1.0EU per 1µg (determined by the LAL method)
Purity : > 95%
Applications : Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
Reconstitution : Reconstitute in PBS or others.
Gene Name : Hsd11b2 hydroxysteroid 11-beta dehydrogenase 2 [ Rattus norvegicus ]
Official Symbol : Hsd11b2
Synonyms : HSD11B2; hydroxysteroid 11-beta dehydrogenase 2; corticosteroid 11-beta-dehydrogenase isozyme 2; 11-DH2; 11-beta-HSD2; 11-beta-hydroxysteroid dehydrogenase type 2; Hydroxysteroid dehydrogenase, 11 beta type 2; NAD-dependent 11-beta-hydroxysteroid dehydrogenase; MGC93528;
Gene ID : 25117
mRNA Refseq : NM_017081
Protein Refseq : NP_058777
UniProt ID : P50233

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (7)

Ask a question
How is HSD11B2 expression regulated in different tissues? 01/26/2021

The expression of HSD11B2 is regulated by various factors in different tissues. In the kidney, where HSD11B2 plays a crucial role in glucocorticoid metabolism, its expression is primarily regulated by aldosterone through the activation of mineralocorticoid receptors. Additionally, other factors, such as angiotensin II and pro-inflammatory cytokines, can also modulate HSD11B2 expression. In tissues outside the kidney, including adipose tissue and the central nervous system, HSD11B2 expression is regulated by a combination of hormonal, metabolic, and inflammatory signals.

What are the implications of altered HSD11B2 activity in metabolic diseases? 08/01/2020

Altered HSD11B2 activity has been implicated in various metabolic diseases. For instance, decreased HSD11B2 activity in adipose tissue has been associated with increased local cortisol levels, contributing to the development of obesity, insulin resistance, and metabolic syndrome. Conversely, increased HSD11B2 activity in other tissues, such as the liver, has been associated with reduced cortisol availability, which may impact glucose metabolism and contribute to the development of hepatic insulin resistance. Understanding the precise mechanisms underlying these associations is crucial for developing targeted therapeutic strategies.

How does HSD11B2 impact the development and progression of cardiovascular diseases? 03/01/2020

HSD11B2 plays a crucial role in cardiovascular health by regulating the balance between cortisol and aldosterone action. Dysregulation of this balance, due to altered HSD11B2 activity, can contribute to the development and progression of cardiovascular diseases. For example, increased cortisol activation of mineralocorticoid receptors in the vasculature can lead to endothelial dysfunction, vascular inflammation, and hypertension. Furthermore, altered HSD11B2 expression in the heart and blood vessels has been associated with cardiac remodeling, fibrosis, and impaired contractility. Understanding the precise mechanisms underlying these effects can provide insights into potential therapeutic interventions.

What are the potential therapeutic strategies targeting HSD11B2? 07/24/2018

Several potential therapeutic strategies targeting HSD11B2 have been explored. These include the development of selective inhibitors that can block the enzyme's activity, leading to increased cortisol availability in specific tissues. Conversely, activators of HSD11B2 could enhance its protective role by promoting cortisol inactivation. Additionally, modulating HSD11B2 expression and activity through lifestyle interventions, such as diet and exercise, may also have therapeutic potential. However, further research is needed to evaluate the efficacy and safety of these strategies in clinical settings.

What is the current understanding of HSD11B2 in the context of stress and mental health? 01/07/2018

The role of HSD11B2 in stress and mental health is an area of active research. Chronic stress has been associated with dysregulation of the hypothalamic-pituitary-adrenal axis and increased cortisol levels, which can influence HSD11B2 activity. Altered HSD11B2 expression and activity in the brain have been implicated in stress-related psychiatric disorders, including depression and anxiety. Furthermore, studies have suggested a potential link between HSD11B2 polymorphisms and susceptibility to stress-related mental health conditions. However, more research is needed to fully elucidate the complex interplay between HSD11B2, cortisol metabolism, and mental health outcomes.

Are there any genetic variations in HSD11B2 associated with disease susceptibility? 12/08/2016

Yes, genetic variations in HSD11B2 have been reported to be associated with disease susceptibility. Single nucleotide polymorphisms (SNPs) in the HSD11B2 gene have been linked to altered enzyme activity and increased risk of developing conditions such as hypertension, cardiovascular disease, and metabolic disorders. These genetic variations can affect HSD11B2 expression, protein stability, or catalytic efficiency, thereby influencing glucocorticoid metabolism and potentially contributing to disease pathogenesis.

What is the physiological role of HSD11B2 in regulating glucocorticoid levels? 08/27/2016

HSD11B2, also known as 11β-hydroxysteroid dehydrogenase type 2, plays a critical role in the regulation of glucocorticoid levels. It acts as a protective enzyme by converting active cortisol into inactive cortisone in mineralocorticoid target tissues, such as the kidney. This enzyme prevents cortisol from binding to the mineralocorticoid receptor, thereby maintaining the selective action of aldosterone on mineralocorticoid receptors. Dysfunction or deficiency of HSD11B2 can lead to excessive cortisol activation of mineralocorticoid receptors, resulting in conditions such as apparent mineralocorticoid excess syndrome.

Customer Reviews (3)

Write a review
Reviews
07/30/2022

    Using this reagent, I am able to better explore the functionality and structure of proteins.

    06/14/2022

      Momentum surges as the experiment unfolds, propelled by the prowess of this exceptional reagent.

      03/12/2020

        With this groundbreaking reagent, experiments unfold effortlessly and joyously.

        Ask a Question for All Hsd11b2 Products

        Required fields are marked with *

        My Review for All Hsd11b2 Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /

        Stay Updated on the Latest Bioscience Trends