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Recombinant Rat Il3 protein, His & GST-tagged

Cat.No. : Il3-643R
Product Overview : Recombinant Rat Il3 aa. (Ile27~Cys166 (Accession # P04823)) fused with N-terminal His & GST tag was produced in E. coli cells.
  • Specification
  • Gene Information
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Source : E. coli
Species : Rat
Tag : His & GST
Form : Freeze-dried powder
Molecular Mass : Predicted Molecular Mass: 45.8kDa
Protein length : Ile27~Cys166 (Accession # P04823)
Endotoxin : <1.0EU per 1µg (determined by the LAL method)
Purity : >90%
Characteristic : The isoelectric point is 7.8.
Applications : SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.
Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Storage buffer : Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative
Reconstitution : Reconstitute in sterile ddH2O.
Gene Name : Il3 interleukin 3 [ Rattus norvegicus (Norway rat) ]
Official Symbol : Il3
Synonyms : IL-3; MCGF; P-cell-stimulating factor; hematopoietic growth factor; mast cell growth factor; multipotential colony-stimulating factor; interleukin-3
Gene ID : 24495
mRNA Refseq : NM_031513.1
Protein Refseq : NP_113701.1
UniProt ID : P04823

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (16)

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What is the difference between IL-3 and GM-CSF In vitro? 04/14/2023

In vitro, IL-3 can induce differentiation and proliferation of myeloid lineage cells and is distinguished from GM-CSF by the relative increase in production of platelets, basophils, and mast cells.

What is the difference in clinical presentation of IL-3 compared to GM-CSF? 04/14/2023

Although effective, IL-3 appears to present no therapeutic advantages compared to G-CSF and GM-CSF, and in direct comparison studies, IL-3 led to a considerably higher rate of adverse events than either of the other cytokines.

Since the structure of IL-3 is different between animals and humans, does it mean that il-3 is not necessary to maintain stable hematopoietic function? 04/14/2023

mice deficient in both bc and IL-3 show the same phenotype as the bc-deficient mice, conclusively demonstrating that, for the most part, the GM-CSF/IL3/IL5 family is not essential for steady-state production of myeloid cells

How does the structure of IL-3 in animals differ from that in humans? 04/14/2023

Unlike in humans, murine IL-3 has a second b chain receptor, bcIL3, which bears 91% homology to the bc chain.

Does loss of cytokine IL-3 affect normal granulocyte, monocyte, and basophil levels? 04/14/2023

Knockout mice were also reported with surprisingly limited phenotypes. Despite the ability of the three cytokines to stimulate hematopoiesis, mice functionally deficient in all three cytokinesdisplay normal circulating levels of granulocytes, monocytes, and basophils.

IL-3 specific receptor subunit CD123 is overexpressed in many hematological malignancies,Is it overexpressed in normal tissues where inflammation occurs? 04/14/2023

non-hematopoietic (e.g., epithelial cells, and fibroblasts) cell subsets can also produce il-3.

When is IL-3 activated by T cells? 04/14/2023

Such as allergic inflammation and parasitic infestation.

Since basophils are the target and source of IL-3, can they regulate the secretion of IL-3 to treat allergic diseases? 04/14/2023

IL-3 can regulate the survival of basophil granulocytes and cytokine production through autocrine, so as to achieve the purpose of treating allergic diseases.

T cell IL-3 expression is specifically regulated by which transcription factor? 04/14/2023

Bhlhe40

Which hematopoietic and non-hematopoietic cell subsets express IL-3? 04/14/2023

E.g., lymphocytes,epithelial cells, and fibroblasts

Are there any completed clinical trials on IL3? 12/20/2021

Yes, there are some completed clinical trials on IL3 results, but the specific results need to refer to the relevant research papers.

What is the effect of IL3 on hematopoietic system? 09/29/2021

This protein can promote stem cell proliferation and differentiation during hematopoietic process, thus increasing the production of different types of blood cells.

Are there any side effects of IL3? 09/24/2019

In general, the IL3 has relatively few side effects, but some unpleasant symptoms such as fever, headache and nausea may occur.

IL3 associated with whether the immune adjustment? 06/28/2019

IL3 can regulate the body's immune response and the function of immune cells, and plays an important role in immune regulation.

What is the solubility and stability of IL3? 05/25/2019

IL3 generally have good solubility and stability, making them easier to handle during drug preparation and storage.

How is the research progress of IL3? 02/27/2019

The study of IL3 is ongoing, including its application in the development of therapeutic drugs, as well as in combination therapy with other immune molecules.

Customer Reviews (3)

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Reviews
06/20/2022

    I've used many brands, but this one is one of my favorites.

    03/08/2021

      The performance is very stable and the price is very reasonable. I would definitely buy this product again.

      12/19/2020

        I have recommended this brand to my colleagues and friends, and they all praise this product.

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