Active Recombinant Rat SELP protein(Met1-Gln700), hFc-tagged

• Cat.No.: SELP-1114R
• Product Overview: Recombinant Rat Selp (AAI01920.1) (Met1-Gln700) was expressed in HEK293, with the Fc region of Human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Rat
• Tag: C-hFc
• Protein length: Met1-Gln700
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells.When 5 x 10E4 cells/well are added to Rat SELP/Fc Chimera coated plates (10 μg/mL with 100 μL/well), > 40% cells will adhere after 1 hour at 37°C.
• Molecular Mass: The recombinant Rat Selp consists 897 amino acids and predicts a molecular mass of 98.7 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Selp selectin P [ Rattus norvegicus ]
• Official Symbol: SELP
• Synonyms: SELP; selectin P; P-selectin; LECAM3; PADGEM; GMP-140; selectin, platelet; granule membrane protein 140; CD62 antigen-like family member P; leukocyte-endothelial cell adhesion molecule 3; platelet activation dependent granule-external membrane protein; PSELECT; MGC124632
• Gene ID: 25651
• mRNA Refseq: NM_013114
• Protein Refseq: NP_037246