| Description : |
SARS Coronavirus is an enveloped virus containing three outer structural proteins, namely the membrane (M), envelope (E), and spike (S) proteins. Spike (S)-glycoprotein of the virus interacts with a cellular receptor and mediates membrane fusion to allow viral entry into susceptible target cells. Accordingly, S-protein plays an important role in virus infection cycle and is the primary target of neutralizing antibodies. |
| Source : |
E. coli |
| Species : |
SARS |
| Tag : |
His |
| Form : |
Sterile filtered and lyophilized from 0.5 mg/ml in 0.05M Acetate buffer pH4. |
| Molecular Mass : |
47.8kDa |
| AA sequence : |
MRGSHHHHHHGMASHMSDNGPQSNQ RSAPRITFGGPTDSTDNNQNGGRNG ARPKQRRPQGLPNNTASWFTALTQH GKEELRFPRGQGVPINTNSGPDDQI GYYRRATRRVRGGDGKMKELSPRWY FYYLGTGPEASLPYGANKEGIVWVA TEGALNTPKDHIGTRNPNNNAATVL QLPQGTTLPKGFYAEGSRGGSQASS RSSSRSRGNSRNSTPGSSRGNSPAR MASGGGETALALLLLDRLNQLESKV SGKGQQQQGQTVTKKSAAEASKKPR QKRTATKQYNVTQAFGRRGPEQTQG NFGDQDLIRQGTDYKHWPQIAQFAP SASAFFGMSRIGMEVTPSGTWLTYH GAIKLDDKDPQFKDNVILLNKHIDA YKTFPPTEPKKDKKKKTDEAQPLPQ RQKKQPTVTLLPAADMDDFSRQLQN SMSGASADSTQA. |
| Purity : |
Greater than 95% as determined by SDS-PAGE. |
| Applications : |
Western blotting. |
| Usage : |
The products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
| Specificity : |
The amino acid sequence of the recombinant SARS-CoV Nucleocapsid protein is 100% homologous to amino acid sequence of the native SARS-CoV Nucleocapsid protein. |
| Solubility : |
Add 0.2 ml of 0.1M Acetate buffer pH4 and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/ml. In higher concentrations the solubility of this antigen is limited. |
| Storage : |
Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. |
