Recombinant SARS S Protein, His-tagged

• Cat.No.: S-671V
• Product Overview: Recombinant S1 subunit of human SARS coronavirus (isolate:WH20) spike (AAX16192.1) (Met1-Arg667) was expressed with a C-terminal polyhistidine tag.

Specification

• Source: Baculovirus-Insect cells
• Species: SARS
• Tag: His
• Predicted N Terminal: Ser 14
• Form: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4, 5%~8% trehalose and mannitol.
• Molecular Mass: The recombinant S1 subunit of human SARS coronavirus (isolate:WH20) spike comprises 665 amino acids and has a predicted molecular mass of 74.4 kDa. The apparent molecular mass of the protein is approximately 85.8 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >90 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: S E2 glycoprotein precursor [ SARS coronavirus ]
• Official Symbol: S
• Synonyms: E2; E2 glycoprotein precursor; spike glycoprotein
• Gene ID: 1489668
• Protein Refseq: NP_828851