|Product Overview:||GST-Synaptobrevin-2 is 331 amino acids in length. This product contains the GST affinity tag and 97 amino acids of the 116 amino acids of the Synaptobrevin-2 sequence. The final 19 amino acids of the native protein, containing the transmembrane domain, are not included in this preparation of Synaptobrevin-2.|
|Description:||The protein was recombinantly expressed in E. coli and purified using affinity and ion exchange chromatography. The GST affinity tag has been retained on the protein.|
|Form:||Each 100 ug vial of recombinant GST-Synaptobrevin-2, when reconstituted with 185 ul of water, contains 100 ug of protein in 20 mM HEPES, pH 7.4, 1.25% lactose.|
|Bio-activity:||The recombinant light chain from botulinum neurotoxin Type D (BoNT/D LC) has been used to test the GST-Synaptobrevin-2 in an endopeptidase assay. Twenty nanomolar BoNT/D LC was incubated with 5 uM GST-Synaptobrevin-2 in 20 mM Tris, pH 8.0, containing 50 mM NaCl and 5 mM DTT at 37 centigrade. In 1 hour, 95% of the GST-Synaptobrevin-2 was shown to be cleaved using SDS PAGE analysis.|
|Molecular Mass:||The molecular weight of the protein is 37.797 kDa based on analysis of the nucleic acid sequence.|
|Protein length:||311 amino acids|
|Purity:||When examined on 4-12% SDS-polyacrylamide gels, this product migrated with an apparent molecular weight of approximately 38 kDa. Densitometric analysis indicates that the protein purity is > 85%.|
|Notes:||GST-Synaptobrevin-2 is not known to be toxic.|
|Storage:||This product is supplied as a lyophilized powder which has been stoppered under vacuum. Store lyophilized vials at 2-8 centigrade. Once dissolved, aliquot and store the product at -20 centigrade. Refrain from multiple freeze/thaw cycles.|
|Concentration:||Protein concentration was determined by absorbance at 280nm using Abs (0.1 %) =1.471. This value is calculated by ProtParamL using an algorithm based on the Edelhoch method with modifications described in Pace et al.
|Warning:||Good laboratory technique should be employed in the safe handling of this product. Wear appropriate laboratory attire including a lab coat, gloves and safety glasses. Nitrile gloves are recommended when handling lyophilized material. This product is intended for research purposes by qualified personnel only. It is not intended for use in humans or as a diagnostic agent.|
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