"PLA2" Related Products

sPLA2 Activity Assay Kit

Cat.No.: Kit-0699
Product Overview: PLA2 (sPLA2) Assay kit provides an accurate and convenient method for measurement of sPLA2 activity. This assay uses the 1,2-dithio analog of diheptanoyl phosphatidylcholine which serves as a substrate for most PLA2s (for example, bee and cobra venoms, pancreatic, etc.) with the exception of cytosolic PLA2. Upon hydrolysis of the thio ester bond at the sn-2 position by PLA2, free thiols are detected using DTNB (5,5-dithio-bis-(2-nitrobenzoic acid)).
Description: Phospholipase A2 (PLA2) catalyzes the hydrolysis of phospholipids at the sn-2 position yielding a free fatty acid and a lysophospholipid. The release of arachidonic acid from membrane phospholipids by PLA2 is believed to be a key step in the control of eicosanoid production within the cell.
Usage: Please read these instructions carefully before beginning this assay. For research use only. Not for human or diagnostic use.
Storage: Stability: 1 year;Storage: -20°C;This kit will perform as specified if stored at -20°C and used before the expiration date indicated on the outside of the box.
Kit Components: sPLA2 Assay Buffer (10X): 1 vial;sPLA2 DTNB: 4 vials;sPLA2 Diheptanoyl Thio-PC (Substrate): 2 vials;Bee venom PLA2 Control: 1 vial;96-Well Plate (Colorimetric Assay): 1 plate;96-Well Cover Sheet: 1 cover.

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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