Col18a1

Case study 1: K A Spivey, 2012

Collagen XXIII is a transmembrane collagen previously shown to be upregulated in metastatic prostate cancer that has been used as a tissue and fluid biomarker for non-small cell lung cancer and prostate cancer. To determine whether collagen XXIII facilitates cancer cell metastasis in vivo and to establish a function for collagen XXIII in cancer progression, collagen XXIII knockdown cells were examined for alterations in in vivo metastasis as well as in vitro cell adhesion.

Loss of collagen XXIII in H460 cells also impaired cell adhesion, anchorage-independent growth and cell seeding to the lung, but did not affect cell proliferation. Corroborating a role for collagen XXIII in cell adhesion, overexpression of collagen XXIII in H1299 cells, which do not express endogenous collagen XXIII, enhanced cell adhesion. This study suggests a potential role for collagen XXIII in mediating metastasis by facilitating cell-cell and cell-matrix adhesion as well as anchorage-independent cell growth.

Fig1. To assess the role of collagen XXIII in cell proliferation, control and knockdown cell lines were seeded in triplicate and grown in complete media for 24, 48, 72, or 96 hours.

Fig2. shRNA-mediated silencing of collagen XXIII alters the expression of proteins involved in cell adhesion and structure.

Case study 2: Chun-Hong Ma, 2004

To explore a safe and efficient strategy of tumor therapy using anti-angiogenetic agents. Endostatin gene with a signal sequence of human IgGgamma chain was amplified by PCR and cloned into pVAX1 plasmid which was the only vector authorized by FDA in clinical trial to construct a recombinant plasmid named as pVAX-sEN. The recombinant plasmid was detected with EcoRI/KpnI and DNA sequencing. To compare the efficiency of gene transfection, expression of endostatin at the treated tumor site was assayed with ELISA.

DNA sequencing showed the endostatin gene with the signal peptide was correctly cloned. In situ gene expression assay indicated that both the ratio of DNA and liposome and the dose of DNA could affect the gene transfection efficiency. Interestingly, naked pVAX-sEN had a similar in situ endostatin expression to pVAX-sEN with liposome. Naked endostatin plasmid intratumoral injection can get a similar gene transfection efficiency to liposome-DNA complex when used in situ.

Fig3. Inhibitory effect of recombinant secretive endostatin on mouse hepatoma cells.

Fig4. In vivo endostatin expression in peripheral blood of treated mice.

Fig1. αEGFR-E-P125A effects on angiogenesis and VM lead to decreased tumor growth and metastasis. (Seung-Uon Shin, 2021)

Fig2. Proposed rES/ID1/TSP-1 signaling pathway. In the absence of rES, ID1 regulates growth factor (BMP and TGF-β) signaling by acting as a transcriptional repressor via dimerization with other basic helix–loop–helix transcription factors. (Alice M. Goyanes, 2020)

Col18a1 involved in several pathways and played different roles in them. We selected most pathways Col18a1 participated on our site, such as Protein digestion and absorption, which may be useful for your reference. Also, other proteins which involved in the same pathway with Col18a1 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

Col18a1 has several biochemical functions, for example, identical protein binding, metal ion binding, protein binding. Some of the functions are cooperated with other proteins, some of the functions could acted by Col18a1 itself. We selected most functions Col18a1 had, and list some proteins which have the same functions with Col18a1. You can find most of the proteins on our site.

Col18a1 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with Col18a1 here. Most of them are supplied by our site. Hope this information will be useful for your research of Col18a1.

TECPR1; PYHIN1; AHI1