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DFFA

  • Official Full Name

    DNA fragmentation factor, 45kDa, alpha polypeptide

  • Overview

    Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  • Synonyms

    DFFA; DNA fragmentation factor, 45kDa, alpha polypeptide; DFF1; ICAD; DFF-45; DNA fragmentation factor subunit alpha; DFF45; inhibitor of CAD; DNA fragmentation factor 45 kDa subunit;

  • Recombinant Proteins
  • Cell & Tissue Lysates
  • Protein Pre-coupled Magnetic Beads
  • Human
  • Mouse
  • Rhesus Macaque
  • Zebrafish
  • E.coli
  • HEK293
  • HEK293T
  • Human
  • In Vitro Cell Free System
  • Mammalian Cell
  • Wheat Germ
  • GST
  • His
  • His (Fc)
  • Avi
  • His|T7
  • Myc
  • DDK
  • N/A
  • N
Species Cat.# Product name Source (Host) Tag Protein Length Price
Human DFFA-2555H Recombinant Human DFFA Protein, GST-tagged Wheat Germ GST
Human DFFA-4316H Recombinant Human DNA Fragmentation Factor, 45kDa, Alpha Polypeptide, His-tagged Human His
Human DFFA-488H Recombinant Human DFFA E.coli N/A
Human DFFA-28396TH Recombinant Human DFFA, His-tagged E.coli His
Human DFFA-27566TH Recombinant Human DFFA, His-tagged E.coli His
Human DFFA-6967HCL Recombinant Human DFFA 293 Cell Lysate HEK293 N/A
Human DFFA-4460H Recombinant Human DFFA Protein, Myc/DDK-tagged, C13 and N15-labeled HEK293T Myc/DDK
Human DFFA-2480HF Recombinant Full Length Human DFFA Protein, GST-tagged In Vitro Cell Free System GST 331 amino acids
Human DFFA-1955H Recombinant Human DFFA Protein (Ala51-Ser305), N-His tagged E.coli N-His Ala51-Ser305
Human DFFA-1956H Recombinant Human DFFA Protein (Met1-Thr331), N-His tagged E.coli N-His Met1-Thr331
Human DFFA-30138H Recombinant Human DFFA protein, GST-tagged E.coli GST Gly8-Gln328
Human DFFA-2817H Recombinant Human DFFA protein, GST-tagged E.coli GST 1-331aa
Mouse DFFA-4531M Recombinant Mouse DFFA Protein Mammalian Cell His
Mouse Dffa-1836M Recombinant Mouse Dffa protein, His & T7-tagged E.coli His/T7 Lys19~Ser262 (Accession # O54786)
Mouse DFFA-2341M Recombinant Mouse DFFA Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Mouse Dffa-2532M Recombinant Mouse Dffa Protein, Myc/DDK-tagged HEK293T Myc/DDK
Mouse DFFA-2341M-B Recombinant Mouse DFFA Protein Pre-coupled Magnetic Beads HEK293
Rhesus Macaque DFFA-1249R Recombinant Rhesus monkey DFFA Protein, His-tagged Mammalian Cell His
Rhesus Macaque DFFA-1074R-B Recombinant Rhesus Macaque DFFA Protein Pre-coupled Magnetic Beads HEK293
Rhesus Macaque DFFA-1074R Recombinant Rhesus Macaque DFFA Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Zebrafish DFFA-742Z Recombinant Zebrafish DFFA Mammalian Cell His
  • Involved Pathway
  • Protein Function
  • Interacting Protein
  • DFFA Related Research Area

DFFA involved in several pathways and played different roles in them. We selected most pathways DFFA participated on our site, such as Activation of DNA fragmentation factor, Apoptosis, Apoptosis Modulation and Signaling, which may be useful for your reference. Also, other proteins which involved in the same pathway with DFFA were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

Pathway Name Pathway Related Protein
Activation of DNA fragmentation factorHMGB2A;HIST1H1A;HIST1H1B;HIST1H1D;H1F0;HIST1H1E;HIST1H1C;HMGB2;DFFA
ApoptosisPRKAR2AB;IKBKG;BID;BNIP3LB;FASLG;BIRC8;TRADD;YWHAQ;DIABLOA
Apoptosis Modulation and SignalingBLK;BOK;PTPN13;DIABLO;BAG3;DFFA;BIK;PTRH2;HRK
Apoptosis induced DNA fragmentationHIST1H1E;HMGB2;HIST1H1D;HIST1H1B;HMGB2A;H1F0;DFFA;HIST1H1C;HIST1H1A
Apoptotic execution phaseHIST1H1E;PLEC;CLSPN;VIML;ADD1;DBNLA;HIST1H1B;BMX;STK26
Caspase cascade in apoptosisCRADD;NUMA1;LMNB1;CFL2;SATB1;GAS2;DIABLO;PARP1;CASP4
FAS pathway and Stress induction of HSP regulationFAF1;DAXX;LMNB2;DFFA
HIV-1 Nef: Negative effector of Fas and TNF-alphaCASP2;CRADD;DAXX;DFFA

DFFA has several biochemical functions, for example, protein binding. Some of the functions are cooperated with other proteins, some of the functions could acted by DFFA itself. We selected most functions DFFA had, and list some proteins which have the same functions with DFFA. You can find most of the proteins on our site.

Function Related Protein
protein bindingFHL1;CPSF4;LLGL1;BCL2L13;PPP1R2P9;SHMT2;P4HA3;PINX1;NRADD

DFFA has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with DFFA here. Most of them are supplied by our site. Hope this information will be useful for your research of DFFA.

DFFB; YWHAG; YWHAZ; HSPB1; Dffb; 7460242

  • Q&As
  • Reviews

Q&As (7)

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What diseases or conditions are associated with dysregulation or abnormal expression of DFFA, particularly in the context of apoptotic dysfunction? 08/28/2019

Dysregulation or abnormal expression of DFFA is associated with various conditions, including cancer and neurodegenerative disorders, highlighting its role in apoptotic dysfunction.

How does DFFA contribute to DNA fragmentation and chromatin condensation during apoptotic cell death? 10/27/2018

DFFA contributes to DNA fragmentation and chromatin condensation by being cleaved and activated by caspases, particularly caspase-3, during apoptotic cell death.

In which cellular compartments is DFFA predominantly localized, especially during the initiation of apoptosis? 10/21/2018

DFFA is predominantly localized in the nucleus, specifically during the initiation of apoptosis, where it actively participates in chromatin condensation and DNA fragmentation.

Are there therapeutic implications or potential targets related to DFFA in diseases involving apoptotic dysregulation and abnormal cell survival? 05/18/2018

There are potential therapeutic implications related to DFFA in diseases involving apoptotic dysregulation and abnormal cell survival, making it a target for drug development.

What is the primary function of DNA Fragmentation Factor Subunit Alpha (DFFA) in cellular processes and apoptosis? 03/05/2018

DNA Fragmentation Factor Subunit Alpha (DFFA) plays a crucial role in cellular processes, primarily contributing to the execution of apoptosis and programmed cell death.

How does DFFA participate in regulating the balance between cell survival and programmed cell death in various tissues? 03/02/2018

DFFA actively participates in regulating the balance between cell survival and programmed cell death in various tissues, influencing tissue homeostasis.

Are there specific caspases or factors that activate DFFA, influencing its role in the execution phase of apoptosis? 04/10/2016

Specific caspases, including caspase-3, activate DFFA, influencing its role in the execution phase of apoptosis and facilitating efficient DNA degradation.

Customer Reviews (3)

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Reviews
12/23/2021

    We felt well-supported throughout our usage of the product, thanks to the dedicated customer service.

    10/18/2019

      Consistency in the product's behavior across experimental conditions enhanced the credibility of our findings.

      05/11/2019

        The product's adaptability to various buffers contributed to its stable and reliable performance.

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