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DnaK

  • Official Full Name

    Chaperone Hsp70

  • Overview

    ATP-regulated binding and release of polypeptide substrates. [More information is available at EcoGene: EG10241]. Hsc56 exhibits specificity toward Hsc62, as Hsc56 does not activate DnaK or Hsc66 ATPase activity .
  • Synonyms

    dnaK; chaperone Hsp70, co-chaperone with DnaJ; ECK0014; groPAB; groPC; groPF; grpC; grpF; JW0013; seg; chaperone Hsp70; DNA biosynthesis; autoregulated heat shock proteins; NP_414555.1;

  • Recombinant Proteins
  • Bacillus subtilis
  • Chlamydia psittaci
  • E.coli
  • Escherichia coli
  • Escherichia coli (strain UTI89 / UPEC)
  • Mycobacterium Tuberculosis
  • Mycoplasma pneumoniae (strain ATCC 29342 / M129)
  • Staphylococcus
  • Staphylococcus aureus
  • Streptomyces coelicolor A3(2)
  • E. coli
  • E. coli or Yeast
  • E.coli
  • Yeast
  • His
  • SUMO
  • N/A
  • N
Species Cat.# Product name Source (Host) Tag Protein Length Price
Bacillus subtilis DNAK-0298B Recombinant Bacillus subtilis DNAK protein, His-tagged E. coli or Yeast His
Chlamydia psittaci dnaK-1010C Recombinant Chlamydia psittaci dnaK Protein (Met1-Asp659), N-His tagged E.coli N-His Met1-Asp659
E.coli DnaK-315E Recombinant E.coli DnaK Chaperone Hsp70, Co-chaperone with DnaJ (385-638) E.coli N/A
E.coli DnaK-313E Recombinant E. coli Dnak Protein E. coli
E.coli DnaK-314E Recombinant E.coli DnaK Chaperone Hsp70, Co-chaperone with DnaJ (1-384) E.coli N/A
E.coli DnaK-312E Recombinant E.coli DnaK Chaperone Hsp70, Co-chaperone with DnaJ (385-546) E.coli N/A
E.coli DnaK-316E Recombinant E.coli DnaK Chaperone Hsp70, Co-chaperone with DnaJ (508-638) E.coli N/A
Escherichia coli dnaK-4455E Recombinant Escherichia coli dnaK protein, His-tagged E.coli His 1-638aa
Escherichia coli (strain UTI89 / UPEC) dnaK-5765E Recombinant Escherichia coli (strain UTI89 / UPEC) dnaK protein, His-tagged Yeast His 1-638aa
Mycobacterium Tuberculosis DnaK-1257H Recombinant Myobacterium Tuberculosis Molecular Chaperone DnaK E.coli N/A
Mycoplasma pneumoniae (strain ATCC 29342 / M129) dnaK-3700M Recombinant Mycoplasma pneumoniae (strain ATCC 29342 / M129) dnaK protein, His-SUMO-tagged E.coli His-SUMO 423-595aa
Staphylococcus DNAK-2955S Recombinant Staphylococcus epidermidis ATCC 12228 DNAK protein, His-tagged E. coli or Yeast His
Staphylococcus DNAK-3518S Recombinant Staphylococcus aureus subsp. aureus NCTC 8325 DNAK protein, His-tagged E. coli or Yeast His
Staphylococcus aureus dnaK-15S Recombinant Staphylococcus aureus dnaK Protein, His-tagged E.coli His
Streptomyces coelicolor A3(2) DNAK-508S Recombinant Streptomyces coelicolor A3(2) DNAK protein, His-tagged E. coli or Yeast His
  • Background
  • Quality Guarantee
  • Case Study
  • Involved Pathway
  • Protein Function
  • Interacting Protein
  • DnaK Related Articles

What is DNAK protein?

DNAK, originally identified for its DNA replication by bacteriophage l in E. coli is the bacterial Hsp70 chaperone. This protein is involved in the folding and assembly of newly synthesized polypeptide chains and in preventing the aggregation of stress-denatured proteins. DNAK is N-terminal ATPase domain and ATP bound to the ATPase domain induces a conformational change in the substrate binding domain (residues385-638). The protein coding region of the ATPase domain of DNAK (amino acids 1-384) was amplified by PCR and cloned into an E. coli expression vector. The DNAK protein is consisted of 384 amino acids and its molecular mass is approximately 41.6 kDa.

What is the function of DNAK protein?

DNAK belongs to the Hsp70 family and is involved in various cellular processes, including protein folding, assembly/disassembly of protein complexes, protein transport, and degradation. Specifically, DNAK assists in the folding of newly synthesized proteins, prevents protein aggregation, and helps in the refolding of denatured or misfolded proteins. It also interacts with other chaperone proteins and co-chaperones to regulate protein folding processes. In addition, DNAK plays a role in cellular stress responses and can be induced by heat shock or other environmental stresses to protect cells from protein damage and promote cell survival.

DNAK related Signaling pathways

There are no specific signaling pathways that have been directly attributed to DNAK protein. However, the activity of DNAK is regulated by various signaling pathways, such as the heat shock response pathway, which is activated in response to cellular stressors like high temperatures, oxidative stress, and exposure to toxins. Additionally, DNAK can be regulated by other signaling pathways involved in protein homeostasis. Furthermore, DNAK can interact with multiple signaling proteins and transcription factors, thereby influencing various cellular processes and signaling cascades indirectly.

DNAK-5.jpg

Fig1. Human Phospho-Kinase Arrays for PC-3 and relative percentage increase of protein phosphorylation after 20 and 60 min of incubation with the exogenous and purified DnaK.

DNAK Related Diseases

No disease has been directly associated with DNAK. DNAK is mainly involved in the process of DNA replication, transcription and repair, and the change of its expression level may affect the growth and differentiation of cells. Therefore, DNAK may be involved in the occurrence and development of certain cancers. For example, several studies have shown that DNAK is expressed at significantly elevated levels in melanoma cells and that its overexpression correlates with tumor aggressiveness and prognosis. In addition, DNAK has also been found to be abnormally expressed in various tumors such as breast cancer and lung cancer.

Bioapplications of DNAK

DNAK protein can be used to develop novel drugs to treat diseases associated with protein folding abnormalities; The expression of DNAK protein is abnormally high in some types of cancer, therefore, DNAK protein can be used as a target for tumor therapy; DNAK protein can also be used to study the pathogenesis of neurodegenerative diseases and the mechanism of heat shock response, and to develop novel therapeutic drugs.

High Purity

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DNAK-2.jpg

Fig2. SDS-PAGE (DNAK-5765E)

Case Study 1: Ping Lu, 2023

DNAK-3.jpg

Fig1. The growth of wild-type (WT) and ΔdnaK C. sakazakii strains, as well as the complementation of dnaK in the ΔdnaK strain (ΔdnaK-C), was monitored in LB medium at pH 7.0.

Case Study 2: Victor Tse, 2024

DNAK-4.jpg

Fig2. SDS-PAGE analysis of rDnaK protein purification by IMAC with imidazole gradient elution.

DnaK involved in several pathways and played different roles in them. We selected most pathways DnaK participated on our site, such as RNA degradation, which may be useful for your reference. Also, other proteins which involved in the same pathway with DnaK were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

Pathway Name Pathway Related Protein
RNA degradationCNOT4B;PABPC1L;XRN2;ENO1A;EXOSC4;WDR61;TOB1;PFKL;XRN1

DnaK has several biochemical functions, for example, . Some of the functions are cooperated with other proteins, some of the functions could acted by DnaK itself. We selected most functions DnaK had, and list some proteins which have the same functions with DnaK. You can find most of the proteins on our site.

Function Related Protein

DnaK has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with DnaK here. Most of them are supplied by our site. Hope this information will be useful for your research of DnaK.

dnaJ; grpE; rne

Hicks, CAE; Willi, B; et al. Protective Immunity against Infection with Mycoplasma haemofelis. CLINICAL AND VACCINE IMMUNOLOGY 22:108-118(2015).
Lee, JJ; Lim, JJ; et al. Characterization of culture supernatant proteins from Brucella abortus and its protection effects against murine brucellosis. COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES 37:221-228(2014).
  • Q&As
  • Reviews

Q&As (5)

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How does DNAK protein interact with other cellular components? 06/03/2022

DNAK interacts with co-chaperones and client proteins, forming complexes that facilitate proper protein folding and function.

How is DNAK protein studied in a clinical laboratory setting? 05/03/2022

Laboratory techniques such as Western blotting and immunohistochemistry are commonly used to analyze DNAK protein expression and localization.

Can DNAK protein be targeted for drug development? 01/09/2022

Efforts are underway to develop drugs that modulate DNAK activity, with the potential for treating diseases associated with protein misfolding.

Is there a link between DNAK protein and cardiovascular diseases? 05/15/2020

Some studies suggest that DNAK may play a role in cardiovascular health, and its dysregulation could contribute to heart-related disorders.

Can DNAK protein be used as a biomarker for treatment response? 10/20/2019

Monitoring changes in DNAK protein levels during treatment may provide insights into the effectiveness of therapeutic interventions.

Customer Reviews (3)

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Reviews
11/23/2021

    Equally noteworthy is the remarkable technical support provided by the manufacturer.

    11/16/2018

      Their team of experts has been instrumental in addressing any issues or concerns I have encountered during my experiments.

      08/22/2018

        The DNAK Protein boasts remarkable stability, ensuring its integrity is maintained during storage and experimentation.

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