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Dhfr

  • Official Full Name

    dihydrofolate reductase

  • Overview

    Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2014]
  • Synonyms

    DHFR; dihydrofolate reductase; DYR; DHFRP1;

  • Recombinant Proteins
  • Cell & Tissue Lysates
  • Protein Pre-coupled Magnetic Beads
  • Assay Kits
  • Chicken
  • Hamster
  • Human
  • Mouse
  • Zebrafish
  • E.coli
  • HEK293
  • HEK293T
  • In Vitro Cell Free System
  • Mammalian Cell
  • Mammalian cells
  • Wheat Germ
  • Flag
  • GST
  • His
  • His (Fc)
  • Avi
  • SUMO
  • Myc
  • DDK
  • N/A
  • N
  • No tag
Species Cat.# Product name Source (Host) Tag Protein Length Price
Human DHFR-375H Recombinant Human Dihydrofolate Reductase, His-tagged E.coli His
Human DHFR-11966H Recombinant Human DHFR, GST-tagged E.coli GST 1-187a.a.
Human DHFR-42H Recombinant Human DHFR protein, His-tagged E.coli His 187aa(including fusion tag)
Human DHFR-28331TH Recombinant Human DHFR, His-tagged E.coli His 187 amino acids
Human DHFR-2586H Recombinant Human DHFR Protein, GST-tagged Wheat Germ GST
Human DHFR-6946HCL Recombinant Human DHFR 293 Cell Lysate HEK293 N/A
Human DHFR-755H Recombinant Human DHFR Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Human DHFR-2818H Recombinant Human DHFR protein, His-SUMO-tagged E.coli His-SUMO 2-187aa
Human DHFR-2526HF Recombinant Full Length Human DHFR Protein, GST-tagged In Vitro Cell Free System GST 187 amino acids
Human DHFR-6261H Recombinant Human DHFR Protein, Myc/DDK-tagged, C13 and N15-labeled HEK293T Myc/DDK
Human DHFR-0618H Recombinant Human DHFR Protein (M1-D187), His tagged E.coli His M1-D187
Human DHFR-1960H Recombinant Human DHFR Protein (Met1-Asp187), N-His tagged E.coli N-His Met1-Asp187
Human DHFR-1590HFL Recombinant Full Length Human DHFR Protein, C-Flag-tagged Mammalian cells Flag
Human DHFR-755H-B Recombinant Human DHFR Protein Pre-coupled Magnetic Beads HEK293
Human DHFR-0617H Recombinant Human DHFR Protein (M1-D187), Tag Free E.coli No tag M1-D187
Mouse Dhfr-3422M Recombinant Mouse Dihydrofolate Reductase, His-tagged E.coli His
Mouse DHFR-4556M Recombinant Mouse DHFR Protein Mammalian Cell His
Mouse Dhfr-7193M Active Recombinant Mouse Dhfr Protein, His-tagged E.coli His 1-187
Mouse DHFR-2354M Recombinant Mouse DHFR Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Mouse Dhfr-2545M Recombinant Mouse Dhfr Protein, Myc/DDK-tagged HEK293T Myc/DDK
Mouse DHFR-2354M-B Recombinant Mouse DHFR Protein Pre-coupled Magnetic Beads HEK293
Hamster DHFR-353HCL Recombinant Human DHFR cell lysate N/A
Zebrafish DHFR-9289Z Recombinant Zebrafish DHFR Mammalian Cell His
Chicken DHFR-1721C Recombinant Chicken DHFR Mammalian Cell His
Kit-2097 Dihydrofolate Reductase Colorimetric Activity Kit N/A
Kit-2100 Dihydrofolate Reductase Inhibitor Screening Kit N/A
  • Involved Pathway
  • Protein Function
  • Interacting Protein
  • Dhfr Related Articles

Dhfr involved in several pathways and played different roles in them. We selected most pathways Dhfr participated on our site, such as Cell Cycle, Cell Cycle, Mitotic, E2F mediated regulation of DNA replication, which may be useful for your reference. Also, other proteins which involved in the same pathway with Dhfr were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

Pathway Name Pathway Related Protein
Cell CycleE2F3;CENPH;ESCO2;HIST4H4;CCNE2;MCM9;SETA;FZR1;ARPP19B
Cell Cycle, MitoticSKA2;DNA2;KIF2C;NIPBL;CEP135;POLD3;TUBGCP4;TUBG2;CDK4
E2F mediated regulation of DNA replication
E2F transcription factor networkYY1;CES5A;HIC1;UXT;CDKN2C;CES3;CES1;E2F4;MYBL2
Fluoropyrimidine ActivitySLC22A7;DHFR;CES1;DPYS;UPP2;DPYD;TYMP
Folate biosynthesisGGH;QDPRB1;ALPPL2;QDPRA;QDPRB2;SPR;MOCS2;ALPP;ALP3
G1/S TransitionCKS1B;DHFR;MYCA
G1/S-Specific Transcription

Dhfr has several biochemical functions, for example, NADPH binding, dihydrofolate reductase activity, drug binding. Some of the functions are cooperated with other proteins, some of the functions could acted by Dhfr itself. We selected most functions Dhfr had, and list some proteins which have the same functions with Dhfr. You can find most of the proteins on our site.

Function Related Protein
NADPH bindingTP53I3;GRHPR;FASN;CBR4;MTRR;KCNAB1;DHFR;HMGCR;QDPR
dihydrofolate reductase activityDHFRL1;DHFR
drug bindingABP1;PYGM;GSTP2;CYP4B1;IL2RA;ADH1;GRIK1;SRP54B;P2RX4
folic acid bindingTYMS;FOLR3;DHFR;DAAM2;FTCD;FOLR1;SLC46A1;MTHFS;FOLR2
mRNA bindingSSB;RBMX;KHSRP;NUDT16;ZFP36L1;DOM3Z;MECP2;DXO;TPR
methotrexate bindingDHFR;FOLR1;DAAM2;FOLR2

Dhfr has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with Dhfr here. Most of them are supplied by our site. Hope this information will be useful for your research of Dhfr.

nleF

Ershler, MA; Olovnikova, NI; et al. Production of IgM monoclonal antibodies in DG44 cells. APPLIED BIOCHEMISTRY AND MICROBIOLOGY 50:819-828(2014).
Nasseri, SS; Ghaffari, N; et al. Increased CHO cell fed-batch monoclonal antibody production using the autophagy inhibitor 3-MA or gradually increasing osmolality. BIOCHEMICAL ENGINEERING JOURNAL 91:37-45(2014).
  • Q&As
  • Reviews

Q&As (5)

Ask a question
Can you name a common DHFR inhibitor drug used in cancer treatment? 03/12/2020

Methotrexate is a common DHFR inhibitor drug used in cancer treatment.

Are there ongoing research efforts to develop new DHFR inhibitors for clinical use? 02/21/2018

Yes, scientists continue to research and develop new DHFR inhibitors with improved efficacy and reduced side effects for various clinical applications.

Are there any drug-resistant strains that have developed due to DHFR inhibitors? 12/06/2017

Yes, drug-resistant strains have emerged due to mutations in DHFR genes, reducing the effectiveness of DHFR inhibitor drugs.

Are there any side effects associated with DHFR inhibitor drugs like Methotrexate? 11/28/2017

Yes, common side effects of DHFR inhibitors include nausea, vomiting, decreased blood cell counts, and liver toxicity.

Can you name an antibiotic that inhibits DHFR in bacteria? 11/21/2016

Trimethoprim is an antibiotic that inhibits DHFR in bacteria.

Customer Reviews (3)

Write a review
Reviews
04/16/2023

    The DHFR protein offered by the manufacturer is of exceptional quality, making it an ideal choice to fulfill my experimental needs.

    03/20/2017

      Another crucial aspect where the manufacturer excels is their efficient supply chain management.

      08/31/2016

        The technical support provided by the manufacturer is invaluable in overcoming experimental hurdles and optimizing the utilization of DHFR protein.

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