Epha4
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Official Full Name
EPH receptor A4
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Overview
This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically h -
Synonyms
EPHA4; EPH receptor A4; EphA4 , TYRO1; ephrin type-A receptor 4; Hek8; EK8; EPH-like kinase 8; TYRO1 protein tyrosine kinase; tyrosine-protein kinase TYRO1; tyrosine-protein kinase receptor SEK; receptor protein-tyrosine kinase HEK8; SEK; TYRO1;
- Recombinant Proteins
- Cell & Tissue Lysates
- Protein Pre-coupled Magnetic Beads
- Chicken
- Cynomolgus Monkey
- Human
- Human
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- Rhesus Macaque
- E.coli
- HEK293
- Human Cell
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- Insect Cell
- Mammalian Cell
- Mammalian cells
- Sf21
- Sf9 Insect Cell
- Wheat Germ
- C
- Fc
- His
- hFc
- DDK|His
- GST
- GST|His
- His (Fc)
- Avi
- His|GST
- N/A
- N
- No tag
- Background
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- Case Study
- Involved Pathway
- Protein Function
- Interacting Protein
- Epha4 Related Articles
What is EPHA4 protein?
EPHA4 (EPH receptor A4) gene is a protein coding gene which situated on the long arm of chromosome 2 at locus 2q36. This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. The EPHA4 protein is consisted of 986 amino acids and its molecular mass is approximately 109.9 kDa.
What is the function of EPHA4 protein?
Upon activation by ephrin ligands, EPHA4 modulates cell morphology and integrin-dependent cell adhesion through regulation of the Rac, Rap and Rho GTPases activity. It plays an important role in the development of the nervous system controlling different steps of axonal guidance including the establishment of the corticospinal projections. In the nervous system, EPHA4 also plays a role in repair after injury preventing axonal regeneration and in angiogenesis playing a role in central nervous system vascular formation. Additionally, its promiscuity makes it available to participate in a variety of cell-cell signaling regulating for instance the development of the thymic epithelium.
EPHA4 Related Signaling Pathway
EPHA4 is involved in many important signaling pathways, which play an important role in cell growth, differentiation, apoptosis, and migration. For example, Ephrin-Eph signaling pathway, ERK signaling pathway, Wnt/β-catenin signaling pathway and so on.
EPHA4 Related Diseases
Due to its important role in signaling pathways, functional abnormalities associated with EPHA4 may lead to a variety of diseases, including neurological developmental disorders (such as congenital brain tumor-like malformations or epilepsy), white matter damage, neurodegenerative diseases (such as Parkinson's disease and Alzheimer's disease), tumors, and more. In addition, mutations in EPHA4 have been linked to a number of human genetic diseases, such as congenital muscular dystrophy and loss of consciousness syndrome.
Fig1. The vicious cycle (surrounded by blue dotted line) was regulated by EphA4-mediated IGF1 synthesis pathway. (Xuefeng Jing, 2016)
Bioapplications of EPHA4
As for the application of EPHA4, although there is currently no clear therapeutic or drug target for EPHA4 treatment, the latest research suggests that EPHA4 may be a potential therapeutic target with the potential to treat neurological diseases and cancer. At the same time, EPHA4 can also be used as a diagnostic indicator to assess the risk and progression of the disease.
High Purity
Fig1. SDS-PAGE (EPHA4-3385H) (PROTOCOL for western blot)
High Bioactivity
Fig2. Activity Data. (EPHA4-3385H)
Case Study 1: Shinichiro Kina, 2023
Fig1. Western blots of OSCC for EphA4 expression and β-actin as a housekeeping control.
Case Study 2: Shinichiro Kina, 2023
Fig2. Differences in the expression of EphA4 in well- and poorly differentiated oral squamous
cell carcinoma (OSCC).
Fig1. An upstream regulator analysis correlated with EphA4 expression identified pathways associated with decreased mTORC1 signaling and T cell activation, including TCR, CD3, CD28, and CD40LG. (Shinichiro Kina, 2023)
Fig2. llustration of the signaling pathways induced in the progeny of colorectal cancer. Unirradiated (left) and irradiated (right) cells. (Priscila Guimarães de Marcondes, 2017)
Epha4 involved in several pathways and played different roles in them. We selected most pathways Epha4 participated on our site, such as Axon guidance, which may be useful for your reference. Also, other proteins which involved in the same pathway with Epha4 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.
Pathway Name | Pathway Related Protein |
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Axon guidance | PHB;RHOA;AP2B1;DUSP9;PLXNB2;LIMK2;SCN3A;RGMB;DUSP2 |
Epha4 has several biochemical functions, for example, ATP binding, DH domain binding, GPI-linked ephrin receptor activity. Some of the functions are cooperated with other proteins, some of the functions could acted by Epha4 itself. We selected most functions Epha4 had, and list some proteins which have the same functions with Epha4. You can find most of the proteins on our site.
Function | Related Protein |
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ATP binding | DCLK1A;HELB;ATP13A;NLRP14;MTHFD1L;C10orf2;DNAJA2L;AK8;P2RX4 |
DH domain binding | |
GPI-linked ephrin receptor activity | EPHA8;EPHA5;EPHA4;EK1;EPHA3;EPHA7 |
PH domain binding | INPP5A;EPHA4;EPB41L2 |
ephrin receptor binding | EFNA1B;EFNA2;PTPN1;NTRK1;EPHA4;ANKS1B;NTRK3;EPHA7;CBL |
identical protein binding | EID3;CLDN17;BNIP2;JMJD6;G6PDX;NLRC4;SUN2;SH3BP4;SERPINA1A |
protein binding | TRPC4AP;PRICKLE1;MPHOSPH6;VIL1;NPEPL1;IQCB1;ZFAND5;CD69;COPS5 |
protein kinase activity | BMPR1BA;PRKG1A;CAD;CSNK1G3;TNNI3K;DCLK1A;RIPK3;DCLK2;SYK |
transmembrane-ephrin receptor activity | EPHA4;EFNA4;EFNB3;EPHA1;EPHB2;EPHA5;EPHB1;EFNA3;EPHA10 |
Epha4 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with Epha4 here. Most of them are supplied by our site. Hope this information will be useful for your research of Epha4.
IKBKG; HSP90AB1
- Q&As
- Reviews
Q&As (7)
Ask a questionEPHA4 is a member of the ephrin receptor family of protein kinases that plays an important role in neural development and synaptic plasticity. It is involved in axon guidance, neuronal migration, and cell proliferation.
EPHA4 plays a critical role in neuronal development as a member of the Eph receptor family, which are receptor tyrosine kinases involved in various cellular processes. Specifically, EPHA4 regulates axon growth and guidance, acting as a repulsive cue to guide axons in the developing nervous system. Its involvement extends to synaptic plasticity and neuronal survival. Dysregulation of EPHA4 has been linked to neurological disorders, including Alzheimer's disease and neuropathic pain. Further research on the precise mechanisms and downstream signaling pathways of EPHA4 will deepen our understanding of its role in neuronal development and potential therapeutic applications.
EPHA4 expression is tightly regulated during nervous system development, with its expression gradually increasing during embryonic development. Additionally, EPHA4 expression is spatially regulated, with high levels in specific regions of the nervous system. Regulation of EPHA4 expression is mediated by several mechanisms, including transcriptional and post-transcriptional mechanisms.
EPHA4 is essential for normal synaptic plasticity, with its activation leading to the modulation of various synaptic proteins, such as AMPA receptors, NMDA receptors, and EphB receptors. Dysregulation of EPHA4-mediated signaling has been implicated in multiple neurodegenerative disorders, affecting both synaptic plasticity and axon growth.
EPHA4 signaling is initiated by binding to its ligands, which leads to activation of downstream signaling pathways. Several signaling pathways are activated by EPHA4, including the MAP kinase pathway, the PI3K-Akt pathway, and the RhoA-ROCK pathway, which play critical roles in the regulation of actin cytoskeleton dynamics.
EPHA4 has emerged as a promising therapeutic target for various neurodegenerative diseases, and several pharmacological inhibitors have been developed to target EPHA4 signaling. These inhibitors have shown efficacy in preclinical models of several neurodegenerative diseases, suggesting their potential in clinical applications.
There is growing evidence that genetic variants in the EPHA4 gene may contribute to the susceptibility to neurodegenerative diseases, such as Alzheimer's and Parkinson's disease. These variants have been shown to modify EPHA4 expression and function, and may contribute to disease pathogenesis via several mechanisms, including synaptic dysfunction and axon degeneration.
Customer Reviews (3)
Write a reviewThe results interpretation process with this protein reagent is straightforward, making it suitable for beginners as well.
The composition ratio of the reagent is highly accurate, ensuring the reliability of the experimental results.
Astounding in both reliability and steadfastness, it confers irrefutable persuasion to the experiment.
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