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SMAD3

  • Official Full Name

    SMAD family member 3

  • Overview

    The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene mothers against decapentaplegic (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009]
  • Synonyms

    SMAD3; SMAD family member 3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436; mothers against decapentaplegic homolog 3; mad3; hMAD-3; hSMAD3; MAD homolog 3; mad homolog JV15-2; mad protein homolog; mothers against DPP homolog 3; SMA- and MAD-related protein 3; SMAD, mothers against DPP homolog 3; MAD, mothers against decapentaplegic homolog 3;

  • Recombinant Proteins
  • Protein Pre-coupled Magnetic Beads
  • Cell & Tissue Lysates
  • Chicken
  • Human
  • Human/Mouse/Rat
  • Mouse
  • Rat
  • Rhesus Macaque
  • E.coli
  • HEK293
  • HEK293T
  • Human
  • Insect Cell
  • Insect Cells
  • Mammalian Cell
  • Mammalian cells
  • Flag
  • GST
  • His
  • His (Fc)
  • Avi
  • His|MBP
  • SUMO
  • Myc
  • DDK
  • Myc|DDK
  • N/A
  • N
Species Cat.# Product name Source (Host) Tag Protein Length Price
Human SMAD3-1094H Recombinant Human SMAD Family Member 3, GST-tagged E.coli GST
Human SMAD3-4599H Recombinant SMAD Family Member 3, His-tagged Human His
Human SMAD3-327H Recombinant Human SMAD3 protein, His/MBP-tagged E.coli His/MBP 241-425 aa
Human SMAD3-6915H Recombinant Human SMAD Family Member 3, His-tagged E.coli His 1-425aa
Human SMAD3-31191TH Recombinant Human SMAD3 E.coli N/A
Human SMAD3-31190TH Recombinant Human SMAD3, His-tagged E.coli His
Human SMAD3-295H Recombinant Human SMAD3 protein, GST-tagged E.coli GST
Human SMAD3-326H Recombinant Human SMAD3 protein, His/MBP-tagged E.coli His/MBP 16-221 aa
Human SMAD3-4788H Recombinant Human SMAD3 Protein, Myc/DDK-tagged, C13 and N15-labeled HEK293T Myc/DDK
Human SMAD3-304H Recombinant Human SMAD3 Protein, His-tagged E.coli His Phe231~Ser425
Human SMAD3-1918H Recombinant Human SMAD3 Protein, MYC/DDK-tagged HEK293 Myc/DDK
Human SMAD3-227HFL Active Recombinant Full Length Human SMAD3 Protein, C-Flag-tagged Mammalian cells Flag
Human SMAD3-2256H Recombinant Human SMAD3 protein, His-tagged Insect Cell His 1-425aa
Human SMAD3-2041H-B Recombinant Human SMAD3 Protein Pre-coupled Magnetic Beads HEK293
Human SMAD3-3506H Recombinant Human SMAD3 protein, His-SUMO-tagged E.coli His-SUMO 1-425aa
Human SMAD3-5867H Recombinant Human SMAD3 Protein (Asp220-Ser425), N-His tagged E.coli N-His Asp220-Ser425
Human SMAD3-1973HF Active Recombinant Full Length Human SMAD3 Protein, GST-tagged E.coli GST 1-425(end)
Human SMAD3-2041H Recombinant Human SMAD3 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Mouse SMAD3-532M Recombinant Mouse Smad3, His-GST tagged Insect Cell GST
Mouse SMAD3-001MCL Recombinant Mouse SMAD3 cell lysate Insect Cell N/A
Mouse Smad3-5951M Recombinant Mouse Smad3 Protein, Myc/DDK-tagged HEK293T Myc/DDK
Mouse Smad3-305M Recombinant Mouse Smad3 Protein, His-tagged E.coli His Phe231~Ser425
Rat SMAD3-5608R Recombinant Rat SMAD3 Protein Mammalian Cell His
Rat SMAD3-5267R Recombinant Rat SMAD3 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Rat SMAD3-5267R-B Recombinant Rat SMAD3 Protein Pre-coupled Magnetic Beads HEK293
Human/Mouse/Rat Smad3-0256H Recombinant Human/Mouse/Rat Smad3 protein, His&GST-tagged Insect Cells His/GST Met1-Ser425
Rhesus Macaque SMAD3-4331R Recombinant Rhesus monkey SMAD3 Protein, His-tagged Mammalian Cell His
Rhesus Macaque SMAD3-4147R Recombinant Rhesus Macaque SMAD3 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Rhesus Macaque SMAD3-4147R-B Recombinant Rhesus Macaque SMAD3 Protein Pre-coupled Magnetic Beads HEK293
Chicken SMAD3-6045C Recombinant Chicken SMAD3 Mammalian Cell His
  • Involved Pathway
  • Protein Function
  • Interacting Protein
  • SMAD3 Related Articles
  • SMAD3 Related Gene Family
  • SMAD3 Related Research Area
  • SMAD3 Related Signal Pathway

SMAD3 involved in several pathways and played different roles in them. We selected most pathways SMAD3 participated on our site, such as FoxO signaling pathway, Cell cycle, Endocytosis, which may be useful for your reference. Also, other proteins which involved in the same pathway with SMAD3 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

Pathway Name Pathway Related Protein
FoxO signaling pathwayTGFBR2;PDPK1;PTEN;TGFBR1A;HOMER1;PRKAG2;AKT1;MAPK3;MAPK8A
Cell cyclePCM1;STAG2;GADD45BB;SKP1;GADD45AA;NDEL1B;SPDL1;ESPL1;ATM
EndocytosisCDC42L2;CXCR2;RAB5C;IL2RA;DDL-2;VPS37A;CAPZA1B;ARRB2B;RAB11BA
Wnt signaling pathwayFZD3A;PLCB3;MAPK10;TCF7L1A;RHOAB;WNT3A;SENP2;AXIN2;WNT11
TGF-beta signaling pathwaySMAD5;PPP2CB;INHBAB;RPS6KB1B;ID3;CHRD;RPS6KB1;BMP2B;RHOAA
Hippo signaling pathwayPPP2R1A;WNT9A;ITGB2L;PPP1CB;TEAD3;STK3;SOX2;LATS2;BMP8A
Adherens junctionPVRL1B;EP300;TGFBR1B;BAIAP2A;SNAI2;PTPRJA;CDC42L;RHOAB;SSX2IP
Signaling pathways regulating pluripotency of stem cellsWNT5B;POU5F1;PCGF6;ESX1;PIK3CD;GSK3B;LHX5;SMAD4;MAP2K2
Chagas disease (American trypanosomiasis)PLCB2;TLR6;MAP2K4;C1QB;CALR;CD3G;TRAF6;MAPK8;TGFBR2
Hepatitis BTGFB2;EGR3;PIK3CA;MAVS;SRC;PRKCA;GRB2;PRKCG;TICAM2
HTLV-I infectionTP53INP1;Pdgfa&Pdgfb;ATF4;MAP2K4;ATF1;SMAD4;RAN;HLA-DQA1;BUB1B
Pathways in cancerTGFB1;IL-8;AHA-1;RASSF1;CBLB;AXIN2;TCEB2;FASLG;BRAF
Colorectal cancerFOS;SMAD3;PIK3R3;RAF1;PIK3R1;AXIN2;KRAS;MSH6;SMAD4
Pancreatic cancerRALGDS;SMAD3;RAC3;VEGFA;SMAD4;RAC2;KRAS;CCND1;MAPK10
Chronic myeloid leukemiaTRP53;IKBKB;HDAC2;CTBP1;SMAD4;MDM2;CRKL;BCR;BRAF
Inflammatory bowel disease (IBD)TNF;HLA-DMB;MAF;IL18;Il23a;IL22;IL23;H2-AB1;IFNGR1

SMAD3 has several biochemical functions, for example, R-SMAD binding, RNA polymerase II activating transcription factor binding, RNA polymerase II core promoter proximal region sequence-specific DNA binding. Some of the functions are cooperated with other proteins, some of the functions could acted by SMAD3 itself. We selected most functions SMAD3 had, and list some proteins which have the same functions with SMAD3. You can find most of the proteins on our site.

Function Related Protein
R-SMAD bindingSMAD6;ZC3H3;ANKRD1;PAX6;SMURF1;FOXH1;TRIM33;SMAD2;PMEPA1
RNA polymerase II activating transcription factor bindingLMO2;EGR2;CREB1;RB1;TBX20;SIN3A;NFE2L2;T;DUSP26
RNA polymerase II core promoter proximal region sequence-specific DNA bindingJUNB;NR1D1;PBX1;MAFB;CDX4;NFIA;TCF3;CREB1;ZNF331
bHLH transcription factor bindingTCF21;USF2;TCF3;MAP3K10;SP1;TCF4;ASCL1;SIRT1;LMO2
beta-catenin bindingTCF7L1B;CALCOCO1;NF2;SMAD7;PTPRK;TRPC4;PARK2;SLC9A3R2;TCF7
chromatin DNA bindingHDAC3;H2AFY;ATOH1;XBP1;PPARGC1A;NKAP;H2AFY2;NEUROG3;SMAD3
co-SMAD bindingSMAD1;TRIM33;SMAD2;FOXH1;CITED1;SMAD3;TGIF1;USP9X;SMAD6
collagen bindingSERPINH1B;PCOLCE2;PCOLCE;CTSS;ITGA3;DDR2;GPR56;MMP13;COMP
core promoter proximal region sequence-specific DNA bindingKLF4;DMRTC2;DMRTC1C2;SMAD3;CXXC1;DMRT3;DMRT2;PAX2;KLF1
enhancer bindingTCF3;MESP1;LEF1;SOX9;SMAD2;SP1;ARNT;TIPARP;RAI1
identical protein bindingPUF60;EPOR;IIGP1;PNMA5;SYP;UHRF1;IAPP;AXIN1;GBP1
phosphatase bindingPPP1R36;SLC9A3R1;PPARA;PPP1R21;SH3RF2;CRY1;KIAA0649;TSC2;CNST
protein bindingMUS81;CHRM3;C4orf19;PHF21A;PLCG1;PLEKHF1;NUP205;SMCO3;SMAD5
protein heterodimerization activityPHC2B;YWHAH;HIST2H3C;TENM1;HIST1H2BK;TAF6L;TAF11;HIST2H2AC;TFDP3
protein homodimerization activityMAP3K10;PRMT2;SOD1;HMGCL;ZHX2;CD8A;MZF1;TRPM8;CHMP4C
protein kinase bindingSMAD1;ERRFI1;PRC1;THY1;CDC25A;RICTOR;CENPJ;PTPRR;CCNE1
sequence-specific DNA bindingIRX5A;FOXO1A;ATF7;BATF;CTCFL;NKX2-1;NR2F6;FOXB1A;FOXC1A
transcription factor activity, RNA polymerase II core promoter sequence-specificMAX;HEY1;SMAD3;PAX7;HEY2;HEYL;TFAP2B;MEF2C;NKX3
contributes_to transcription factor activity, protein bindingSMAD4;SMAD3
transcription factor activity, sequence-specific DNA bindingZNF831;PBX1;PRDM2;RNF4;RORAB;FUBP1;BATF;MTA3;MEF2C
contributes_to transcription factor activity, sequence-specific DNA bindingFOXH1;SMAD3;SOX19B;SOX1B;VDRB;HNRNPAB;TAF10;SMAD4;VDR
transcription factor bindingPDX1;ADD1;AGTR2;TCF12;HTT;ERCC3;MED12;UBE2I;ID1
transcription regulatory region DNA bindingFOXC2;MLL1;CCNT1;TP63;HOXA13;FOS;SERPINB1A;RFX3;HNRNPL
transforming growth factor beta receptor bindingAMH;INHBC;GDF1;INHBA;LEFTY1;SMAD2;GDF6;BMP3;BMP8B
transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activitySMAD1;SMAD2;SMAD3;SMAD9
ubiquitin bindingSQSTM1;TOM1L1;NPLOC4;VPS28;USP5;FAF1;CRY1;C36E6.2;PER2
ubiquitin protein ligase bindingUBOX5;PRKAR2A;KDM4A;XRCC5;CUL1A;UBE2N;ARRB2;TRAF4;WASH
zinc ion bindingESRRB;RNF148;KLF4;RNF217;DTX3;SIRT2;ADH5;MICAL2B;ADAMTS15A

SMAD3 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with SMAD3 here. Most of them are supplied by our site. Hope this information will be useful for your research of SMAD3.

SMAD4; SKI

Smithrithee, R; Niyonsaba, F; et al. Human beta-defensin-3 increases the expression of interleukin-37 through CCR6 in human keratinocytes. JOURNAL OF DERMATOLOGICAL SCIENCE 77:46-53(2015).
McMillin, M; Galindo, C; et al. Gli1 activation and protection against hepatic encephalopathy is suppressed by circulating transforming growth factor beta 1 in mice. JOURNAL OF HEPATOLOGY 61:1260-1266(2014).
TGF
  • Q&As
  • Reviews

Q&As (6)

Ask a question
What is the method used to determine the expression level and activity of SMAD3 protein? 12/05/2022

The expression level of SMAD3 protein can be detected by immunohistochemistry, western blotting, real-time PCR and other techniques. At the same time, the activity of SMAD3 protein can be assessed by detecting the phosphorylation level and the amount of SMAD3 protein within the nucleus.

How are the interactions between SMAD3 protein and other proteins regulated? 05/25/2022

interaction between SMAD3 protein and other proteins is regulated by a variety of factors, including phosphorylation, methylation, acetylation and other modifications, and interactions with other proteins. These factors can affect the interaction between SMAD3 protein and other proteins, and then affect the regulation process of TGF-β signal transduction pathway.

How is SMAD3 protein involved in the TGF-β signaling pathway? 04/28/2022

In the TGF-β signaling pathway, SMAD3 protein is activated by TGF-β to form complexes with other SMAD proteins, and then enters the nucleus to regulate the expression of specific genes, thereby affecting the growth, differentiation, apoptosis and immune response of cells.

How is the expression level of SMAD3 protein regulated? 03/05/2022

expression level of SMAD3 protein is regulated by a variety of factors, including gene transcription, translation, modification, and degradation. These factors can affect the expression level of SMAD3 protein, which in turn affects the growth, differentiation and apoptosis of cells.

What is the main function of SMAD3 protein? 01/25/2022

The main function of SMAD3 protein is to transmit signals in the TGF-β signal transduction pathway and regulate cell growth, differentiation, apoptosis and immune response.

What is SMAD3 protein? 07/22/2020

SMAD3 protein is a protein that plays a key role in intracellular signal transduction and belongs to the SMAD family. It is an effector molecule in the transforming growth factor-β (TGF-β) signaling pathway.

Customer Reviews (3)

Write a review
Reviews
05/14/2021

    High expression level, good stability, suitable for high precision experiments.

    03/08/2021

      Compared with other brands I have used, this brand is more stable and reliable.

      01/29/2021

        Strong antibacterial activity, not easy to be contaminated.

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