WT1
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Official Full Name
Wilms tumor 1
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Overview
Wilm’s Tumor (WT), a sporadic and familial childhood kidney tumor, is genetically heterogeneous. Wilm’s tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The WT1 gene is normally expressed in fetal kidney and mesothelioma. -
Synonyms
WT1; Wilms tumor 1; GUD; Wilms tumor protein; AWT1; WAGR; WIT 2; amino-terminal domain of EWS|last three zinc fingers of the DNA-binding domain of WT1; WT33; NPHS4; WIT-2; EWS-WT1;
- Recombinant Proteins
- Cell & Tissue Lysates
- Protein Pre-coupled Magnetic Beads
- Chicken
- Human
- Mouse
- Rat
- E.coli
- HEK293
- Insect Cell
- Mammalian Cell
- Sf9 Insect Cell
- Yeast
- Flag
- His
- His (Fc)
- Avi
- Myc
- His|GST
- SUMO
- N/A
- N
- Background
- Quality Guarantee
- Case Study
- Involved Pathway
- Protein Function
- Interacting Protein
- WT1 Related Articles
- WT1 Related Research Area
Fig1. WT1 is an important factor for AML maintenance in multiple subtypes. (Sandeep Potluri, 2021)
What is WT1 protein?
WT1 (WT1 transcription factor) gene is a protein coding gene which situated on the short arm of chromosome 11 at locus 11p13. The WT1 gene is normally expressed in fetal kidney and mesothelioma. WT1, the product of WT1 gene, is a nuclear protein with structural motifs characteristic of transcription factors, including four C-terminal zinc fingers. While different pre-mRNA processing could result in 16 isoforms of the protein, the inclusion or exclusion of exon 5 and three amino acids (KTS) between zinc fingers 3 and 4 largely affects the activity of the WT1 protein. The WT1 protein is consisted of 449 amino acids and its molecular mass is approximately 49.2 kDa.
What is the function of WT1 protein?
WT1 is a transcription factor that plays an important role in cellular development and cell survival. With the inclusion of exon 5, WT-1 (+KTS) binds to both DNA and RNA and is RNase but not DNase sensitive. This form also colocalizes with splicing factor in a speckled nuclear particle, suggesting that WT-1 protein may function as both a transcription factor and a splicing regulator. At the same time, it has a tumor suppressor as well as an oncogenic role in tumor formation.
WT1 Related Signaling Pathway
The protein encoded by WT1 gene is a bidirectional transcription factor, which has dual functions of inhibiting tumor growth and activating oncogene transcription. WT1 may negatively regulate the Wnt/β-catenin signaling pathway, and is also regulated by many signaling pathways, such as PI3K/Akt and MAPK/ERK signaling pathways. It is also involved in transcriptional regulation of some genes as a transcription factor.
WT1 Related Diseases
Wilm's tumor (WT1) is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. Mutations in the WT1 gene are also associated with frasier syndrome (FS). FS is an X-linked genetic disorder in which mutations in the WT1 gene cause tubular dysplasia and gonadal hypoplasia. Other related diseases include: nephroblastoma, skeletal system diseases, reproductive system diseases, etc. In addition, it is highly expressed in all types of solid tumors and leukemias, especially acute myeloid leukemia (AML).
Bioapplications of WT1
WT1 can be used as a tumor marker to diagnose and monitor the effect of tumor therapy. In addition, anti-sense oligonucleotides, siRNA and other drugs targeting WT1 are also being studied and are expected to become a new tumor therapy.
High Purity
Fig1. SDS-PAGE (WT1-1402H) (PROTOCOL for western blot)
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Fig2. SDS-PAGE (WT1-5272H) (PROTOCOL for western blot)
Case Study 1: Chunhong Wang, 2022
Fig1. Overexpression of miR-671-5p impairs but knockdown of miR-671-5p protects podocyte integrity in vitro.
Case Study 2: Zhuna Wu, 2023
Fig2. Significantly high WT1 expression was observed in cervical caner tissues with relapse.
Fig2. Schematic presentation depicting the mechanisms of KH-type splicing regulatory protein (KSRP) in promoting the progression of clear cell renal cell carcinoma (ccRCC) via targeting neural precursor cell-expressed developmentally downregulated 4 like (NEDD4L). (Yi-Chieh Yang, 2023)
WT1 involved in several pathways and played different roles in them. We selected most pathways WT1 participated on our site, such as Transcriptional misregulation in cancer, which may be useful for your reference. Also, other proteins which involved in the same pathway with WT1 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.
| Pathway Name | Pathway Related Protein |
|---|---|
| Transcriptional misregulation in cancer | TRAF1;H3F3C;BIRC3;EYA1;MMP3;ITGB7;MLLT3;HIST1H3F;IGFBP3 |
WT1 has several biochemical functions, for example, C2H2 zinc finger domain binding, RNA binding, protein binding. Some of the functions are cooperated with other proteins, some of the functions could acted by WT1 itself. We selected most functions WT1 had, and list some proteins which have the same functions with WT1. You can find most of the proteins on our site.
| Function | Related Protein |
|---|---|
| C2H2 zinc finger domain binding | EHMT1;GATA1;EHMT2;TAF9;LEF1;HMGA2;EBF1;THAP7;ZXDC |
| RNA binding | EIF4B;CELF2;AGFG1;LIN28A;SYNCRIP;THOC6;EIF4G1A;RPS7;HENMT1 |
| protein binding | CPNE1;MTF1;GBP2;FOXJ2;GRIN2B;WHSC2;POLD3;CAMK1;IL15RA |
| sequence-specific DNA binding | PPARGC1A;HOXA9A;HOXD10A;IRX6A;ESRRGB;PBX3B;IRX4A;MSXD;EVE1 |
| transcription factor activity, sequence-specific DNA binding | ZFP161;LHX8A;HOMEZB;NFE2L2A;CREMB;E2F8;POU2F1A;KLF12;ZGPAT |
| transcription regulatory region DNA binding | SALL1A;NFE2L2;TFAP2A;WNT1;SALL1;TAF9;NKX2;ZFP746;RFX3 |
| transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding | ETV1;TFAP2C;OTX2;TCF12;NKX2-8;GATA1;POU4F2;ONECUT2;CDX4 |
| zinc ion binding | TRIM60;BRPF1;PRKCG;ARHGEF2;HERC2;ZDHHC16A;MMP25;CAR1;ADAMTSL5 |
WT1 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with WT1 here. Most of them are supplied by our site. Hope this information will be useful for your research of WT1.
KRTAP10-8; KRT40; DVL3; NPM3; ZNF205; DIRAS3; NAT2; CCL5; KLK7; MDM2; TAOK1; CASZ1; THRSP
- Q&As
- Reviews
Q&As (5)
Ask a questionTargeted therapies and immunotherapies aimed at inhibiting or modulating WT1 expression are being investigated for their potential in cancer treatment.
Apart from Wilms' tumor, WT1 is examined in leukemia, mesothelioma, and some solid tumors as a diagnostic marker.
Yes, understanding the WT1 status in a patient's cancer can guide the development of personalized treatment plans, potentially improving outcomes.
Yes, there are several clinical trials exploring the role of WT1 as a therapeutic target and its potential in cancer treatment.
High levels of WT1 expression are often associated with poor prognosis in various cancers, indicating a more aggressive disease course.
Customer Reviews (3)
Write a reviewIt has been widely employed in protein electron microscopy structure analysis, where it has demonstrated remarkable efficacy.
Broad applicability make it a valuable asset for a wide range of research endeavors.
Researchers can rely on the WT1 Protein to deliver reliable and reproducible results, facilitating robust data interpretation and scientific advancements.
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