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Thiol Fluorometric Detection Kit

Cat.No. : Kit-0816
Product Overview : Thiol Fluorometric Detection Kit provides an ultrasensitive fluorimetric assay to quantitate thiol content that exists either in a small molecule or on a protein. The proprietary non-fluorescent dye used in the kit becomes strongly fluorescent upon reacting with thiol. The kit can detect as little as 1 picomole of cysteine or GSH in a 100 µL assay volume (10 nM). The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. The thiol sensor used in the kit generates a strongly fluorescent adduct upon reacting with a thiol compound. The resulted adduct has the spectral proterties almost identical to those of fluorescein. In addition, both absorption and emission spectra of the thiol adduct are pH-independent, making this assay kit highly robust. The signal can be easily read by a fluorescence microplate reader at Ex/Em = 490/520 nm.
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Publication :
An aminotransferase is responsible for the deamination of the N-terminal leucine and required for formation of oxazolone ring A in methanobactin of Methylosinus trichosporium OB3b. (2017)

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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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