Urine Albumin-to-Creatinine Ratio Assay Kit

• Cat.No.: Kit-2161
• Product Overview: UACR Assay Kit provides a simple, sensitive, and high-throughput adaptable assay that detects albumin (detection range: 0.02- 2.5 mg/ml), creatinine (detection range: 0.002 -0.5 mg/ml) and urine albumin-to-creatinine ratio. The UACR ratio is determined in two steps: First, albumin is determined by using a probe (AB580) that specifically recognizes albumin (Ex/Em = 600/630 nm). Second, creatinine is converted to sarcosine via enzymatic reactions. Sarcosine is specifically oxidized generating a product that reacts with a probe producing a chromophore that can be detected at 570 nm.

Specification

• Size: 100 assays
• Applications: Estimation of albumin in biological samplesEstimation of creatinine in biological samplesDetermination of UACR in mammalian urine samples
• Storage: Store kit at -20°C, protected from light. Briefly spin small vials prior to opening. Read entire protocol before performing the assay.Creatinine Assay Buffer, Albumin Assay Buffer, and Albumin Diluent: Store at -20°C. Bring to room temperature (RT) before use.Albumin Probe (AB580) and Creatinine Probe: Light sensitive. Store at -20°C. Bring to RT before use.Creatinase, Creatininase, and Creatinine Enzyme Mix: Reconstitute with 220 µl of Creatinine Assay Buffer. Aliquot and store at - 20°C. Freeze/thaw should be limited to one time. Keep on ice during use.BSA Standard (2 mg/ml): Store at RT.Creatinine (10 μmol): Reconstitute with 115 µl of dH2O to generate 10 µg/µl Creatinine Standard. Dissolve completely. Store at -20°C. Use within 2 months.