Yeast Expression Systems (P pastoris / S cerevisiae)
Utilizing Creative Biomart's proprietary YeastXceedTM Technology, selected stable and durable production strains that resemble mammalian system are employed for high-yield, high-productivity protein processing, thus dramatically reducing the associated costs and inherent time requirements.
HostOptimTM boosts recombinant protein production per cell by increasing the protein per mRNA ratio across a range of eukaryotic production hosts. Other available recombinant production technologies typically target transcription and are limited to specific production systems. HostOptimTM can be used to increase the production yield of your recombinant protein and can be combined with transcription enhancing or other production improving technologies.
Yeast protein expression system is the most economical eukaryotic expression system for both secretion and intracellular expression. It is ideally suitable for large-scale production of recombinant eukaryotic proteins.
Our expertise in protein expression spans both P. pastoris
and S. cerevisiae
expression systems. If you're looking for protein production via a fed-batch pichia processes, we have the experienced and credentialed staff to move your project forward.
Our YeastXceedTM Technology considers promoters, optimal copies of vector per cell, inducibility, signal peptides, and cellular location, which are essential forhigh-level recombinant protein production.
Creative Biomart's engineering-enhanced protein expression technologies enable a high success rate of over 95% over a wide variety of recombinant proteins, and our proprietary YeastXceedTM technology is pushing yields even higher. Many secretory proteins that bacterial expression systems yield as insoluble or as inactive inclusion bodies can be produced successfully by our yeast expression system using native or yeast secretion signals.
We have identified DNA elements which are instrumental in increasing translation rates when included in recombinant protein expression vectors. These elements are functional across eukaryotic production platforms, including mammalian cells, yeast, and most fungal systems. Based on these elements, Creative Biomart has developed its proprietary HostOptimTM technology.
Using a range of mammalian, yeast and fungal cell types, the functionality of HostOptimTM technology has been confirmed for a variety of industry relevant platforms. Depending on initial expression levels, expression of secreted recombinant proteins can be increased several-fold, and is coupled to a similar increase in recombinant protein to mRNA ratios.