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Active Recombinant Human SIRPA protein, His/Avi-tagged, Biotinylated

Cat.No. : SIRPA-052H
Product Overview : Biotinylated Recombinant Human SIRPA(Glu31-Asn371) protein, fused to His/Avi tag at the C-terminus, was expressed in CHO cells .
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  • Gene Information
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Description : Signal regulatory protein alpha (SIRP alpha, designated CD172a), also called SHPS-1 (SHP substrate 1) and previously, MyD-1 (Myeloid/Dendritic-1), is a monomeric ~90 kDa type I transmembrane glycoprotein that belongs to the SIRP/SHPS (CD172) family of the immunoglobulin superfamily (1-4). SIRPs are paired receptors, with similar extracellular domains but differing C-termini and functions (1, 2). The 503 amino acid (aa) human SIRP alpha contains a 342 aa extracellular domain (ECD), with one V-type, and two C1 type Ig domains, and three potential N glycosylation sites. It has a 110 aa cytoplasmic sequence with ITIM motifs that recruit tyrosine phosphatases SHP-1 and SHP-2 when phosphorylated (4). Human SIRP alpha has more than 40 described polymorphisms, including the prominent BIT (Brain Ig like molecule with Tyrosine-based activation motifs, also called SIRP alpha 2 or PTPNS) (5). One reported isoform lacks aa 1-101, which eliminates most of the V type Ig domain. Human SIRP alpha ECD shares 61%, 60%, 71%, 72% and 73% aa identity with mouse, rat, porcine, bovine and equine SIRP alpha, respectively; it shares 84% and 76% aa identity with human SIRP beta 1 and SIRP gamma, respectively (2). SIRP alpha is expressed mainly on myeloid cells, including macrophages, neutrophils, dendritic and Langerhans cells (3-6). It is also found on neurons, smooth muscle and endothelial cells (7-9). SIRP alpha shows adhesion to the ubiquitous CD47/IAP (integrin associated protein), while SIRP gamma binds more weakly and SIRP alpha 1 does not bind at all (1, 2). Mouse and human SIRP alpha -CD47 binding only cross-reacts for specific polymorphisms and influences engraftment of xenotransplanted stem cells (6, 10). SIRP alpha engagement generally produces a negative regulatory signal (4). Low SIRP alpha recognition of CD47, which occurs on aged erythrocytes or platelets or xenogenic cells, promotes clearance of CD47low cells from circulation (11, 13). SIRP alpha recognition of surfactants SP-A and SP-D in the lung can inhibit alveolar macrophage cytokine production (14). The CD47 integrin-SIRP alpha interaction is reported to promote macrophage fusion during osteoclastogenesis (15).
Source : CHO cells
Species : Human
Tag : His/Avi
Predicted N Terminal : Glu31
Form : Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Bio-activity : Measured by its binding ability in a functional ELISA.
When Recombinant Human CD47 Fc Chimera is immobilized at 1 µg/mL (100 µL/well), Biotinylated Recombinant Human
SIRPA His-tag Avi-tag binds with an ED50 of 0.2-1.4 μg/mL.
Molecular Mass : 49-62 kDa, under reducing conditions
Protein length : Glu31-Asn371
Endotoxin : <0.10 EU per 1 μg of the protein by the LAL method.
Purity : >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Applications : Bioactivity
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution : Reconstitute at 500 μg/mL in PBS.
Gene Name : SIRPA signal-regulatory protein alpha [ Homo sapiens ]
Official Symbol : SIRPA
Synonyms : SIRPA; signal-regulatory protein alpha; protein tyrosine phosphatase, non receptor type substrate 1 , PTPNS1; tyrosine-protein phosphatase non-receptor type substrate 1; BIT; CD172a; MFR; MYD 1; P84; SHPS 1; SHPS1; SIRP; SIRP ALPHA 1; SIRPalpha; SIRPalpha2; myd-1 antigen; inhibitory receptor SHPS-1; macrophage fusion receptor; CD172 antigen-like family member A; tyrosine phosphatase SHP substrate 1; brain-immunoglobulin-like molecule with tyrosine-based activation motifs; MYD-1; CD172A; PTPNS1;
Gene ID : 140885
mRNA Refseq : NM_001040022
Protein Refseq : NP_001035111
MIM : 602461
UniProt ID : P78324

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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09/30/2020

    I am thankful for the immense support provided by this protein reagent for my experimental work! 

    06/14/2020

      This protein substance is truly an indispensable tool in my laboratory!

      09/03/2019

        Its performance is stable and reliable, and the manufacturer's quality control ensures that every experiment runs smoothly.

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