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Aflibercept Bioassay Kit

Cat.No. : Kit-1613
Product Overview : Aflibercept Bioassay kits provide a functional, robust, highly sensitive, and easy-to-use cell-based assay to study aflibercept potency and neutralizing antibodies. The bioassay kits contain all the reagents needed for a complete assay including cells, detection reagents, cell plating reagent, positive control agonist, and assay plates. The pre-validated, frozen cells have been manufactured for single-use and are provided in a ready-to-assay format that saves time and adds convenience.
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Storage : HEK 293 KDR/KDR Bioassay Cells
Cells are shipped on dry ice and should arrive in a frozen state. To ensure maximum cell viability, thaw the vials as soon as possible upon receipt.
If continued storage of the frozen vials is necessary, store as follows:
ƒ Short term (24 hours or less): Store vials at -80°C immediately upon arrival. (DO NOT store at -80°C for more than 24 hours).
ƒ Long term (greater than 24 hours): Vials should ONLY be stored in the vapor phase of liquid nitrogen (LN2).
Safety Warning: A face shield, gloves, and lab coat should be worn at all times when handling frozen vials. The manufacturer of the
cryovial recommends storing the vials in the vapor phase above the LN2. Upon thawing, if LN2 is present in the cryovial, it rapidly
converts back to its gas phase which can result in the explosion of the vial upon its removal from the LN2 tank.

Bioassay Detection Kit
Store at -20°C. Once thawed, the detection reagents can be kept at 4°C for up to 4 days. For longer storage (up to the expiration date listed in the kit certificate of analysis), the reagent should be aliquoted and stored at -20°C until needed. Avoid multiple freeze-thaw cycles.
To make aliquots suitable for testing one assay plate each, 1mL of Detection Reagent 1 per aliquot can be dispensed and frozen down. 4mL of Detection Reagent 2 per aliquot can be dispensed and frozen down separately. Do not mix the two reagents during aliquoting.

AssayComplete Cell Plating Reagent
Once thawed, the Cell Plating Reagent can be stored at 4°C for up to 4 weeks. For longer storage (up to the expiration date listed in the kit certificate of analysis), the reagent should be aliquoted and stored at -20°C until needed. Avoid multiple freeze-thaw cycles. To make aliquots suitable for testing one assay plate each, 30mL of reagent per aliquot can be dispensed and frozen down.

Protein Dilution Buffer
Once thawed, the Protein Dilution Buffer can be stored at 4°C for up to 4 weeks. For longer storage (up to the expiration date listed in the kit certificate of analysis), the reagent should be aliquoted and stored at -20°C until needed. Avoid multiple freeze-thaw cycles. To make aliquots suitable for testing one assay plate each, 10 mL of reagent per aliquot should be dispensed and frozen down. This amount may vary depending on your stock sample concentrations and should be adjusted accordingly.

Recombinant Human VEGF165 Control Agonist
Store at -20°C until ready to use. Centrifuge the vial prior to opening to maximize recovery. Reconstitute to a concentration of 100 μg/mL by adding 100 μL of Protein Dilution Buffer. Reconstituted ligand is stable for 12 months at -20 to -80°C, or 1 week at 2-8 °C.

96-well Tissue Culture Treated Plates
Store at room temperature.
Size : 2 x 96-well

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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What biomarkers or imaging techniques can be used to assess the response to Aflibercept treatment, and how can they be validated experimentally? 04/12/2022

Biomarkers or imaging techniques, such as blood-based biomarkers or imaging modalities like MRI or PET, can be used to assess the response to Aflibercept treatment and validated through clinical studies.

Can Aflibercept be combined with other therapeutic agents or treatment modalities to enhance its anti-angiogenic or anti-tumor effects, and how can these synergistic effects be studied experimentally? 09/25/2021

Aflibercept can be combined with other therapeutic agents or treatment modalities to enhance its anti-angiogenic or anti-tumor effects, and these synergistic effects can be studied through combination therapy experiments in preclinical models or clinical trials.

What are the mechanisms of action of Aflibercept in inhibiting angiogenesis or blocking vascular endothelial growth factor (VEGF) signaling pathways? 04/02/2021

Aflibercept inhibits angiogenesis and blocks VEGF signaling pathways by sequestering VEGF ligands and preventing their interaction with their receptors.

Can Aflibercept be modified or engineered to improve its pharmacokinetic properties, enhance its therapeutic efficacy, or reduce potential side effects, and how can these modifications be evaluated experimentally? 08/23/2020

Aflibercept can be modified or engineered to improve its pharmacokinetic properties, enhance therapeutic efficacy, or reduce side effects, and these modifications can be evaluated through in vitro and in vivo experiments to assess their impact on Aflibercept's function.

What are the downstream signaling events triggered by Aflibercept binding, and how can these signaling pathways be studied experimentally? 04/19/2018

Downstream signaling events triggered by Aflibercept binding can be studied using techniques such as Western blotting, immunoprecipitation, or gene expression analysis.

How does Aflibercept protein interact with its target molecules, and what is the binding affinity or specificity? 03/04/2018

Aflibercept protein interacts with its target molecules through specific binding interactions, and the binding affinity or specificity can be determined using techniques such as surface plasmon resonance or isothermal titration calorimetry.

How does Aflibercept affect tumor growth or progression, and can its anti-tumor activity be assessed using in vitro or in vivo models? 11/06/2015

Aflibercept affects tumor growth or progression by inhibiting angiogenesis and can be evaluated using in vitro cell-based assays or in vivo tumor xenograft models.

What are the optimal dosing regimens and treatment schedules for Aflibercept, and how can its efficacy be evaluated in clinical trials or preclinical models? 10/20/2015

Optimal dosing regimens and treatment schedules for Aflibercept can be determined through dose-finding studies in clinical trials or preclinical efficacy studies.

Are there any known side effects or toxicities associated with Aflibercept treatment, and how can they be monitored or mitigated experimentally? 06/11/2015

Side effects or toxicities associated with Aflibercept treatment can be monitored and mitigated through careful patient monitoring, dose adjustments, or combination therapies.

What are the pharmacokinetic properties of Aflibercept, such as its half-life, bioavailability, and distribution in different tissues? 06/04/2015

The pharmacokinetic properties of Aflibercept, including its half-life, bioavailability, and tissue distribution, can be determined through pharmacokinetic studies in animal models or clinical trials.

Customer Reviews (3)

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Reviews
07/26/2021

    Unraveling protein-protein interactions in chromatin remodeling for epigenetic modifications.

    05/20/2019

      Investigating protein-protein interactions in viral replication for antiviral strategies.

      09/20/2018

        Mapping protein-protein interactions in DNA transcription for gene expression control.

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