BLK Activity Assay Kit

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BLK Activity Assay Kit

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Cat.No. : Kit-1566
Product Overview : CTK Activity cell lines are engineered to the catalytic domain from CTK of interest fused to the cytokine receptor binding domain (CRBD) of a JAK kinase. The cells co-an Enzyme Acceptor (EA) tagged SH2 domain and a ProLink (PK) tagged Cytokine receptor. The CRBD is designed to bind the CTK catalytic domain to the receptor-PK. Activation of the cytokine receptor-PK induces receptor phosphorylation by the CTK catalytic domain. This leads to SH2-EA recruitment, forcing complementation of the two β-galactosidase enzyme fragments (EA and PK). The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent signal. These cells have been modified to prevent long term propagation and expansion using a proprietary compound that has no apparent effect on assay performance.
Size : 100 dp(1 x 96-well)/200 dp(2 x 96-well)/1000 dp(10 x 96-well)
Storage : Short term (2 weeks): Store in vapor phase of liquid N2

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