The development of ELISA is based on the immobilization and enzyme labeling of antigen or antibody. The immunological activity of antigen or antibody remains after the immobilization. And enzyme-labeled antigen or antibody remains both their immunological activity and enzymatic activity. Materials: Antigen  Serum Reagent and kit: Carbonate coating buffer  PBST  BSA Equipment and Supplies: 96-Well ELISA plates  4℃refrigerator  Incubator  Spectrophotometer Procedure:  (1) Coating antigen 1. Dilute the antigen into 10-20μg/ml with 50 mM carbonate coating buffer (pH9.6)…