Principle and Protocol of Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis (SDS-PAGE)

Principle The concentration of polyacrylamide gels can be prepared as required in two electrophoresis systems —called “continuous system” and “discontinuous system”. The biggest feature of “discontinuous system” lies in its greatly improved sample separation resolution. Main features of this electrophoresis are: (1) Use of two gel systems with different concentrations; (2) Solution composition and pH are different for the preparation of the two gel and are also different from electrophoresis…

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