|Description:||Early screening of a drug candidate"s potential to induce or inhibit CYP1A1/2 is a critical step in the drug development process. The CYP1A1/2 Induction Reporter Assay Kit is a novel method to assess CYP1A2 induction. Our Reverse Transfection Reporter Assays have overcome many of the disadvantages of other transfection approaches. The CYP1A1/2 Induction Reporter Assay Kit consists of a 96-well plate coated with a transfection complex containing a Secreted Alkaline Phosphatase (SEAP) reporter construct regulated by the human CYP1A1/2 gene promoter. Cells grown on the CYP1A1/2 Reverse Transfection Strip Plate will introduce the reporter gene into the nucleus. Binding of inducer-activated endogenous transcription factors to the CYP1A1/2 promoter initiates a signal resulting in expression of SEAP which is secreted into the cell culture medium. Aliquots of medium are removed at time intervals beginning at about 24 hours and SEAP activity is measured simply by adding a luminescence-based alkaline phosphatase substrate provided in the kit. The kit is simple to use and can be easily adapted to high-throughput screening for potential CYP1A1 and CYP1A2 inducers.|
|Kit Components:||CYP1A1/2 Reporter Reverse Transfection Strip Plate: 1 plate; -20°C3-Methylcholanthrene Positive Control: 1 vial/40 µl; -20°CSEAP Substrate (Luminescence): 1 vial/15 ml; 4°C96-Well Solid Plate (white) with lid: 3 plates; Room Temperature|
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